Lin, Ziqi, Zhang, Chenlong, Zhang, Xiaoxin, Shi, Na, Wen, Yongjian, Han, Chengxia, Du, Dan, Liu, Tingting, Jin, Tao, Deng, Lihui et al (show 9 more authors)
(2020)
Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2020 (1).
9189457-.
Abstract
Protein kinase C-potentiated inhibitor protein of 17 kDa (CPI-17), a specific inhibitor of myosin light-chain phosphatase (MLCP) regulated by proinflammatory cytokines, is central for calcium sensitisation. We investigated the effects of chaiqin chengqi decoction (CQCQD) on the CPI-17/MLCP pathway in the small intestinal smooth muscle cells (SMCs) and strips (SMS) in an AP model. Necrotising AP was induced in rats by intraperitoneal injections (IPI) of L-ornithine (3.0 g/kg, pH 7.0; hourly × 2) at 1 hour apart; controls received saline. In treatment groups, carbachol (CCh; 60 <i>μ</i>g/kg, IPI) or CQCQD (20 g/kg; 2-hourly × 3, intragastric) was administered. The necrotising AP model was associated with systemic inflammation (serum IL-1<i>β</i> and TNF-<i>α</i>) and worsened jejunum histopathology and motility (serum vasoactive intestinal peptide and intestinal fatty acid-binding protein) as the disease progressed. There was decreased intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>) SMCs. Contractile function of isolated SMCs was reduced and associated with down-regulated expression of key mRNAs and proteins of the CPI-17/MLCP pathway as well as increased IL-1<i>β</i> and TNF-<i>α</i>. CQCQD and CCh significantly reversed these changes and the disease severity. These data suggest that CQCQD can improve intestinal motility by modulating the CPI-17/MLCP pathway in small intestinal smooth muscle during AP.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 3208 Medical Physiology, 32 Biomedical and Clinical Sciences, Digestive Diseases, 2.1 Biological and endogenous factors, Oral and gastrointestinal |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 16 Jul 2021 08:16 |
| Last Modified: | 12 Aug 2024 05:24 |
| DOI: | 10.1155/2020/9189457 |
| Open Access URL: | https://doi.org/10.1155/2020/9189457 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3130241 |
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