Evaluation of intranasal nafamostat or camostat for SARS-CoV-2 chemoprophylaxis in Syrian golden hamsters.

Neary, M ORCID: 0000-0002-4960-2139, Box, H, Sharp, J, Tatham, L ORCID: 0000-0001-9448-8876, Curley, P ORCID: 0000-0003-4596-2708, Herriott, J, Kijak, E, Arshad, U ORCID: 0000-0003-1586-1885, Hobson, JJ ORCID: 0000-0003-2551-1774, Rajoli, Rkr ORCID: 0000-0002-6015-5712
et al (show 8 more authors) (2021) Evaluation of intranasal nafamostat or camostat for SARS-CoV-2 chemoprophylaxis in Syrian golden hamsters. [Preprint]

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Successful development of a chemoprophylaxis against SARS-CoV-2 could provide a tool for infection prevention implementable alongside vaccination programmes. Camostat and nafamostat are serine protease inhibitors that inhibit SARS-CoV-2 viral entry in vitro but have not been characterised for chemoprophylaxis in animal models. Clinically, nafamostat is limited to intravenous delivery and while camostat is orally available, both drugs have extremely short plasma half-lives. This study sought to determine whether intranasal dosing at 5 mg/kg twice daily was able to prevent airborne transmission of SARS-CoV-2 from infected to uninfected Syrian golden hamsters. SARS-CoV-2 viral RNA was above the limits of quantification in both saline- and camostat-treated hamsters 5 days after cohabitation with a SARS-CoV-2 inoculated hamster. However, intranasal nafamostat-treated hamsters remained RNA negative for the full 7 days of cohabitation. Changes in body weight over the course of the experiment were supportive of a lack of clinical symptomology in nafamostat-treated but not saline- or camostat-treated animals. These data are strongly supportive of the utility of intranasally delivered nafamostat for prevention of SARS-CoV-2 infection and further studies are underway to confirm absence of pulmonary infection and pathological changes.

Item Type: Preprint
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 21 Jul 2021 11:26
Last Modified: 18 Jan 2023 21:35
DOI: 10.1101/2021.07.08.451654
Open Access URL: https://www.google.com/search?client=firefox-b-e&q...
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3130833