Owen, Siân 
ORCID: 0000-0001-5330-3177, Wenner, Nicolas, Dulberger, Charles, Rodwell, Ella, Bowers-Barnard, Arthur, Quinones-Olvera, Natalia, Rigden, Daniel ORCID: 0000-0002-7565-8937, Rubin, Eric ORCID: 0000-0001-5120-962X, Garner, Ethan ORCID: 0000-0003-0141-3555, Baym, Michael ORCID: 0000-0003-1303-5598 et al (show 1 more authors)
(2020)
Prophage-encoded phage defense proteins with cognate self-immunity.
Cell Host and Microbe.
2020.07.13.199331-.
ISSN 1931-3128
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Abstract
<h4>Summary</h4> Temperate phages are pervasive in bacterial genomes, existing as vertically-inherited islands called prophages. Prophages are vulnerable to the predation of their host bacterium by exogenous phages. Here we identify BstA, a novel family of prophage-encoded phage defense proteins found in diverse Gram-negative bacteria. BstA drives potent suppression of phage epidemics through abortive infection. During lytic replication, the bstA -encoding prophage is not itself inhibited by BstA due to a self-immunity mechanism conferred by the anti-BstA ( aba ) element, a short stretch of DNA within the bstA locus. Inhibition of phage replication by distinct BstA proteins from Salmonella, Klebsiella and Escherichia prophages is functionally interchangeable, but each possesses a cognate aba element. The specificity of the aba element ensures that immunity is exclusive to the replicating prophage, and cannot be exploited by heterologous BstA-encoding phages. BstA allows prophages to defend host cells against exogenous phage attack, without sacrificing their own lytic autonomy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 3107 Microbiology, 3207 Medical Microbiology, 32 Biomedical and Clinical Sciences, 31 Biological Sciences, Genetics, Biodefense, Emerging Infectious Diseases, Infectious Diseases, 2.1 Biological and endogenous factors, Infection |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 06 Aug 2021 07:11 |
| Last Modified: | 07 Dec 2024 11:30 |
| DOI: | 10.1101/2020.07.13.199331 |
| Open Access URL: | https://www.biorxiv.org/content/10.1101/2020.07.13... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3132485 |
Available Versions of this Item
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Prophage-encoded phage defense proteins with cognate self-immunity. (deposited 30 Jul 2020 13:19)
- Prophage-encoded phage defense proteins with cognate self-immunity. (deposited 06 Aug 2021 07:11) [Currently Displayed]
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