Temporal, Spatial, and Genomic Analyses of Enterobacteriaceae Clinical Antimicrobial Resistance in Companion Animals Reveals Phenotypes and Genotypes of One Health Concern

Singleton, David A ORCID: 0000-0002-1980-5410, Pongchaikul, Pisut ORCID: 0000-0001-8757-3554, Smith, Shirley, Bengtsson, Rebecca J, Baker, Kate ORCID: 0000-0001-5850-1949, Timofte, Dorina ORCID: 0000-0002-7261-738X, Steen, Stephen, Jones, Matthew, Roberts, Larry, Sánchez-Vizcaíno, Fernando
et al (show 5 more authors) (2021) Temporal, Spatial, and Genomic Analyses of Enterobacteriaceae Clinical Antimicrobial Resistance in Companion Animals Reveals Phenotypes and Genotypes of One Health Concern. Frontiers in Microbiology, 12. p. 2160.

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<sec>BackgroundAntimicrobial resistance (AMR) is a globally important one health threat. The impact of resistant infections on companion animals, and the potential public health implications of such infections, has not been widely explored, largely due to an absence of structured population-level data.</sec><sec>ObjectivesWe aimed to efficiently capture and repurpose antimicrobial susceptibility test (AST) results data from several veterinary diagnostic laboratories (VDLs) across the United Kingdom to facilitate national companion animal clinical AMR surveillance. We also sought to harness and genotypically characterize isolates of potential AMR importance from these laboratories.</sec><sec>MethodsWe summarized AST results for 29,330 canine and 8,279 feline Enterobacteriaceae isolates originating from companion animal clinical practice, performed between April 2016 and July 2018 from four VDLs, with submissions from 2,237 United Kingdom veterinary practice sites.</sec><sec>ResultsEscherichia coli (E. coli) was the most commonly isolated Enterobacteriaceae in dogs (69.4% of AST results, 95% confidence interval, CI, 68.7–70.0) and cats (90.5%, CI 89.8–91.3). Multi-drug resistance was reported in 14.1% (CI 13.5–14.8) of canine and 12.0% (CI 11.1–12.9) of feline E. coli isolates. Referral practices were associated with increased E. coli 3rd generation ≤ cephalosporin resistance odds (dogs: odds ratio 2.0, CI 1.2–3.4). We selected 95 E. coli isolates for whole genome analyses, of which seven belonged to sequence type 131, also carrying the plasmid-associated extended spectrum β-lactamase gene bla<sub>CTX–M–</sub><sub>15</sub>. The plasmid-mediated colistin resistance gene mcr-9 was also identified for the first time in companion animals.</sec><sec>ConclusionsLinking clinical AMR data with genotypic characterization represents an efficient means of identifying important resistance trends in companion animals on a national scale.</sec>

Item Type: Article
Uncontrolled Keywords: antimicrobial resistance, companion animal, surveillance, digital health, Escherichia coli, one health
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 23 Aug 2021 08:08
Last Modified: 18 Jan 2023 21:33
DOI: 10.3389/fmicb.2021.700698
Open Access URL: https://www.frontiersin.org/articles/10.3389/fmicb...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3134355