Metastasis-inducing protein S100A4 interacts with p53 in the nuclei of living cells

Liu, Z, Meng, Y, Wang, H, Rudland, PS ORCID: 0000-0002-7491-0846, Barraclough, R ORCID: 0000-0002-7203-1194 and Zhang, S ORCID: 0000-0002-9869-8026 (2019) Metastasis-inducing protein S100A4 interacts with p53 in the nuclei of living cells Precision Radiation Oncology, 3 (1). pp. 23-28. ISSN 2398-7324, 2398-7324

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Abstract

Objective: S100A4 can bind to p53 in vitro. However, it is unclear whether the proteins interact in the nuclei of living cells. Methods: Initially, using an optical biosensor, the ability of CFP-S100A4 to bind with p53-YFP was examined in vitro. The fluorescence resonance energy transfer (FRET) technique using laser confocal microscopy was applied to detect the interaction of S100A4 with p53 in living cells. Results: Imaging FRET in HeLa cells expressing S100A4 and p53 showed an increase in cyan fluorescent protein fluorescence intensity, after photobleaching yellow fluorescent protein in cell nuclei. In comparison, the imaging of cell nuclei expressing mutant S100A4-C and p53 or S100A4 and yellow fluorescent protein as the negative control showed the occurrence of FRET, and demonstrated that the interaction between S100A4 and p53 occurred in the nuclei of living HeLa cells. However, FRET did not occur in the cytoplasm of cells, showing that S100A4 did not interact with p53 in the cytoplasm. Conclusion: S100A4 can interact directly with p53 in the nuclei of living cells. This might be a molecular basis for metastasis-inducing S100A4 in vivo. The results also suggest that the inhibition of the calcium-binding site of S100A4 might be a possible anticancer therapy.

Item Type:	Article
Uncontrolled Keywords:	3101 Biochemistry and Cell Biology, 32 Biomedical and Clinical Sciences, 31 Biological Sciences, Cancer, 2.1 Biological and endogenous factors
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	26 Aug 2021 07:45
Last Modified:	22 Jan 2026 09:12
DOI:	10.1002/pro6.63
Related Websites:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3134710
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