O'Loughlin, Danielle
(2021)
Using nanotopography and macromolecular crowding to influence in vitro collagen deposition in a corneal stroma model
PhD thesis, University of Liverpool.
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Abstract
The corneal stroma is a transparent and avascular tissue constituting 90% of the cornea. The stroma comprises collagenous lamellae, oriented roughly orthogonally, and is populated by quiescent keratocytes. In vitro models of the stroma can be used for a variety of applications from drug toxicity to investigating the mechanisms of collagen fibril alignment. There are several drawbacks of current models: the orientation of collagen is not always considered; cell sheets are often manually stacked, a technically challenging and unreliable method; and cells typically require an extended culture period (several months) to create a construct that resembles the stroma. This project aimed to overcome these problems by providing cells with topographical cues to induce the deposition of an aligned matrix, with stratification and rotation of the deposited collagens. Macromolecular crowding (MMC) was incorporated into the model to accelerate collagen deposition. The aim of this study was to develop an in vitro model that faithfully resembled the corneal stroma. Corneal stromal stem cells (hCSSCs), human corneal fibroblasts (hCFs) and human bone marrow mesenchymal stromal cells (hMSCs) were isolated, characterised and assessed ... (continues)
| Item Type: | Thesis (PhD) |
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| Divisions: | Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Sep 2021 15:21 |
| Last Modified: | 02 Nov 2024 10:19 |
| DOI: | 10.17638/03136510 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3136510 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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