Sadouki, Zahra, McHugh, Timothy D, Aarnoutse, Rob, Canseco, Julio Ortiz, Darlow, Christopher 
ORCID: 0000-0002-5400-3413, Hope, William ORCID: 0000-0001-6187-878X, van Ingen, Jakko, Longshaw, Christopher, Manissero, Davide, Mead, Andrew et al (show 8 more authors)
(2021)
Application of the hollow fibre infection model (HFIM) in antimicrobial development: a systematic review and recommendations of reporting.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 76 (9).
pp. 2252-2259.
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Application of the hollow fibre infection model (HFIM) in antimicrobial development a systematic review and recommendations .pdf - Published version Download (742kB) | Preview |
Abstract
<h4>Objectives</h4>This systematic review focuses on the use of the in vitro hollow fibre infection model (HFIM) for microbial culture. We summarize the direction of the field to date and propose best-practice principles for reporting of the applications.<h4>Methods</h4>Searches in six databases (MEDLINE®, EMBASE®, PubMed®, BIOSIS®, SCOPUS® and Cochrane®) up to January 2020 identified 129 studies meeting our inclusion criteria. Two reviewers independently assessed and extracted data from each publication. The quality of reporting of microbiological and technical parameters was analysed.<h4>Results</h4>Forty-seven out of 129 (36.4%) studies did not report the minimum pharmacokinetic parameters required in order to replicate the pharmacokinetic profile of HFIM experiments. Fifty-three out of 129 (41.1%) publications did not report the medium used in the HFIM. The overwhelming majority of publications did not perform any technical repeats [107/129 (82.9%)] or biological repeats [97/129 (75.2%)].<h4>Conclusions</h4>This review demonstrates that most publications provide insufficient data to allow for results to be evaluated, thus impairing the reproducibility of HFIM experiments. Therefore, there is a clear need for the development of laboratory standardization and improved reporting of HFIM experiments.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Anti-Infective Agents, Anti-Bacterial Agents, Reproducibility of Results, Reference Standards |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 13 Sep 2021 09:22 |
| Last Modified: | 21 Feb 2023 16:15 |
| DOI: | 10.1093/jac/dkab160 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3136942 |
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