Stewart, Alexander, Sinclair, Emma, Ng, Joseph 
ORCID: 0000-0002-3617-5211, O’Hare, Joselli Silvia, Page, Audrey, Serangeli, Ilaria, Margreitter, Christian, Kasar, Nora, Longman, Katherine, Frampas, Cecile et al (show 12 more authors)
(2021)
Pandemic, epidemic, endemic: B cell repertoire analysis reveals unique anti-viral responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus.
[Preprint]
Abstract
Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of IGHV4-39 in both EBOV and COVID-19 infection cohorts. We also show unique characteristics absent in RSV infection or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risks off-target effects.
| Item Type: | Preprint |
|---|---|
| Uncontrolled Keywords: | 3207 Medical Microbiology, 32 Biomedical and Clinical Sciences, 3204 Immunology, Vaccine Related, Infectious Diseases, Emerging Infectious Diseases, Prevention, Coronaviruses Disparities and At-Risk Populations, Biodefense, Genetics, Clinical Research, Coronaviruses, Rare Diseases, Biotechnology, Immunization, 2.1 Biological and endogenous factors, Infection, 3 Good Health and Well Being |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 22 Oct 2021 07:01 |
| Last Modified: | 01 Aug 2024 18:44 |
| DOI: | 10.1101/2021.08.19.456951 |
| Open Access URL: | https://doi.org/10.1101/2021.08.19.456951 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3141193 |
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