Antibacterial, Remineralising and Matrix Metalloproteinase Inhibiting Scandium-doped Phosphate Glasses for Treatment of Dental Caries



Valappil, Sabeel P ORCID: 0000-0001-9646-8406, Abou Neel, Ensanya A, Pickup, David M, Burden, Emily, Sahdev, Rohan, Miles, Emma J, Cooper, Lee, Ansari, Tahera I, Hanna, John V and Higham, Susan M ORCID: 0000-0002-4097-4702
(2021) Antibacterial, Remineralising and Matrix Metalloproteinase Inhibiting Scandium-doped Phosphate Glasses for Treatment of Dental Caries. Dental Materials, 38 (1). pp. 94-107.

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Abstract

<h4>Objectives</h4>Antibiotic resistance is increasingly a growing global threat. This study aimed to investigate the potential use of newly developed scandium-doped phosphate-based glasses (Sc-PBGs) as an antibacterial and anticariogenic agent through controlled release of Sc<sup>3+</sup> ions.<h4>Methods</h4>Sc-PBGs with various calcium and sodium oxide contents were produced and characterised using thermal and spectroscopic analysis. Degradation behaviour, ion release, antibacterial action against Streptococcus mutans, anti-matrix metalloproteinase-2 (MMP-2) activity, remineralisation potential and in vivo biocompatibility were also investigated.<h4>Results</h4>The developed glass system showed linear Sc<sup>3+</sup> ions release over time. The released Sc<sup>3+</sup> shows statistically significant inhibition of S. mutans biofilm (1.2 log<sub>10</sub> CFU reduction at 6 h) and matrix metalloproteinase-2 (MMP-2) activity, compared with Sc-free glass and positive control. When Sc-PBGs were mounted alongside enamel sections, subjected to acidic challenges, alternating hyper- and hypomineralisation layers consistent with periods of re- and demineralisation were observed demonstrating their potential remineralising action. Furthermore, Sc-PBGs produced a non-toxic response when implanted subcutaneously for 2 weeks in Sprague Dawley rats.<h4>Significance</h4>Since Sc<sup>3+</sup> ions might act on various enzymes essential to the biological mechanisms underlying caries, Sc-PBGs could be a promising therapeutic agent against cariogenic bacteria.

Item Type: Article
Uncontrolled Keywords: Scandium-doped phosphate-based glasses, Antibacterial, MMP-2, Remineralisation and, biocompatibility
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Dentistry
Depositing User: Symplectic Admin
Date Deposited: 01 Nov 2021 08:38
Last Modified: 18 Jan 2023 21:25
DOI: 10.1016/j.dental.2021.10.014
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3142276