Fothergill, Joanne 
ORCID: 0000-0002-7012-1508, Floyd, Rachel and Newman, John
(2022)
Invasion and diversity in Pseudomonas aeruginosa urinary tract infections.
Journal of Medical Microbiology, 71 (3).
001458-.
ISSN 0022-2615, 1473-5644
Abstract
Introduction. P. aeruginosa is an opportunistic Gram-negative pathogen frequently isolated in urinary tract infections (UTI) affecting elderly and catheterized patients and associated with ineffective antibiotic treatment and poor clinical outcomes. Gap statement. Invasion has been shown to play an important role in UTI caused by E. coli but has only recently been studied with P. aeruginosa. The ability of P. aeruginosa to adapt and evolve in chronic lung infections is associated with resistance to antibiotics but has rarely been studied in P. aeruginosa UTI populations. Aim. We sought to determine whether phenotypic and genotypic heterogeneity exists in P. aeruginosa UTI isolates and whether, like urinary pathogenic Escherichia coli, these could invade human bladder epithelial cells – two factors that could complicate antibiotic treatment. Methodology. P. aeruginosa UTI samples were obtained from five elderly patients at the Royal Liverpool University Hospital as part of routine diagnostics. Fourty isolates from each patient sample were screened for a range of phenotypes. The most phenotypically diverse isolates were genome sequenced. Gentamicin protection assays and confocal microscopy were used to determine capacity to invade bladder epithelial cells. Results. Despite significant within-patient phenotypic differences, no UTI patient was colonized by distinct strains of P. aeruginosa. Limited genotypic differences were identified in the form of non-synonymous SNPs. Gentamicin protection assays and confocal microscopy provided evidence of P. aeruginosa’s ability to invade bladder epithelial cells. Conclusions. Phenotypic variation and cell invasion could further complicate antibiotic treatment in some patients. More work is needed to better understand P. aeruginosa UTI pathogenesis and develop more effective treatment strategies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Urinary tract infection, antibiotic resistance, invasion, heterogeneity, intracellular |
| Divisions: | Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology Faculty of Health & Life Sciences > School of Health Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 02 Nov 2021 11:43 |
| Last Modified: | 28 Feb 2026 23:39 |
| DOI: | 10.1099/jmm.0.001458 |
| Open Access URL: | https://doi.org/10.1099/jmm.0.001458 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3142494 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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