Stomatal Responses to Light, CO₂, and Mesophyll Tissue in <i>Vicia faba</i> and <i>Kalanchoe fedtschenkoi</i>

Santos, Mauro G, Davey, Phillip A, Hofmann, Tanja A, Borland, Anne, Hartwell, James ORCID: 0000-0001-5000-223X and Lawson, Tracy (2021) Stomatal Responses to Light, CO₂, and Mesophyll Tissue in <i>Vicia faba</i> and <i>Kalanchoe fedtschenkoi</i>. FRONTIERS IN PLANT SCIENCE, 12. 740534-.

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Abstract

The responses of stomatal aperture to light intensity and CO₂ concentration were studied in both <i>Vicia faba</i> (C₃) and <i>Kalanchoë fedtschenkoi</i> (Crassulacean acid metabolism; CAM), in material sampled from both light and dark periods. Direct comparison was made between intact leaf segments, epidermises grafted onto exposed mesophyll, and isolated epidermal peels, including transplantations between species and between diel periods. We reported the stomatal opening in response to darkness in isolated CAM peels from the light period, but not from the dark. Furthermore, we showed that C₃ mesophyll has stimulated CAM stomata in transplanted peels to behave as C₃ in response to light and CO₂. By using peels and mesophyll from plants sampled in the dark and the light period, we provided clear evidence that CAM stomata behaved differently from C₃. This might be linked to stored metabolites/ions and signalling pathway components within the guard cells, and/or a mesophyll-derived signal. Overall, our results provided evidence for both the involvement of guard cell metabolism <i>and</i> mesophyll signals in stomatal responses in both C₃ and CAM species.

Item Type:	Article
Uncontrolled Keywords:	stomata, CAM, mesophyll, Vicia faba, Kalanchoe fedtschenkoi, conductance, stomatal dynamics
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	08 Nov 2021 08:13
Last Modified:	06 Oct 2023 17:06
DOI:	10.3389/fpls.2021.740534
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3142874