Genetically Determined Serum 25-Hydroxyvitamin D Is Associated with Total, Trunk, and Arm Fat-Free Mass: A Mendelian Randomization Study.



Kirwan, R ORCID: 0000-0003-4645-0077, Isanejad, M ORCID: 0000-0002-3720-5152, Davies, IG ORCID: 0000-0003-3722-8466 and Mazidi, M
(2022) Genetically Determined Serum 25-Hydroxyvitamin D Is Associated with Total, Trunk, and Arm Fat-Free Mass: A Mendelian Randomization Study. The journal of nutrition, health & aging, 26 (1). pp. 46-51.

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Abstract

<h4>Purpose</h4>Low serum vitamin D status has been associated with reduced muscle mass in observational studies although the relationship is controversial and a causal association cannot be determined from such observations. Two-sample Mendelian randomization (MR) was applied to assess the association between serum vitamin D (25(OH)D) and total, trunk, arm and leg fat-free mass (FFM).<h4>Methods</h4>MR was implemented using summary-level data from the largest genome-wide association studies (GWAS) on vitamin D (n=73,699) and total, trunk, arm and leg FFM. Inverse variance weighted method (IVW) was used to estimate the causal estimates. Weighted median (WM)-based method, and MR-Egger, leave-one-out were applied as sensitivity analysis.<h4>Results</h4>Genetically higher serum 25(OH)D levels had a positive effect on total (IVW = Beta: 0.042, p = 0.038), trunk (IVW = Beta: 0.045, p = 0.023) and arm (right arm IVW = Beta: 0.044, p = 0.002; left arm IVW = Beta: 0.05, p = 0.005) FFM. However, the association with leg FFM was not significant (right leg IVW = Beta: 0.03, p = 0.238; left leg IVW = Beta: 0.039, p = 0.100). The likelihood of heterogeneity and pleiotropy was determined to be low (statistically non-significant), and the observed associations were not driven by single SNPs. Furthermore, MR pleiotropy residual sum and outlier test did not highlight any outliers.<h4>Conclusions</h4>Our results illustrate the potentially causal, positive effect of serum 25(OH)D concentration on total, trunk and upper body appendicular fat-free mass.

Item Type: Article
Uncontrolled Keywords: Leg, Arm, Adipose Tissue, Humans, Vitamin D, Body Composition, Polymorphism, Single Nucleotide, Genome-Wide Association Study, Mendelian Randomization Analysis, Torso
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User: Symplectic Admin
Date Deposited: 15 Nov 2021 10:09
Last Modified: 08 Jan 2024 05:02
DOI: 10.1007/s12603-021-1696-1
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3143189