Glycation of Host Proteins Increases Pathogenic Potential of Porphyromonas gingivalis

Smiga, Michal, Smalley, John W, Slezak, Paulina, Brown, Jason L, Sieminska, Klaudia, Jenkins, Rosalind E, Yates, Edwin A ORCID: 0000-0001-9365-5433 and Olczak, Teresa (2021) Glycation of Host Proteins Increases Pathogenic Potential of Porphyromonas gingivalis INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22 (21). 12084-. ISSN 1661-6596, 1422-0067

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Abstract

The non-enzymatic addition of glucose (glycation) to circulatory and tissue proteins is a ubiquitous pathophysiological consequence of hyperglycemia in diabetes. Given the high incidence of periodontitis and diabetes and the emerging link between these conditions, it is of crucial importance to define the basic virulence mechanisms employed by periodontopathogens such as Por-phyromonas gingivalis in mediating the disease process. The aim of this study was to determine whether glycated proteins are more easily utilized by P. gingivalis to stimulate growth and promote the pathogenic potential of this bacterium. We analyzed the properties of three commonly encoun-tered proteins in the periodontal environment that are known to become glycated and that may serve as either protein substrates or easily accessible heme sources. In vitro glycated proteins were characterized using colorimetric assays, mass spectrometry, far-and near-UV circular dichroism and UV–visible spectroscopic analyses and SDS-PAGE. The interaction of glycated hemoglobin, serum albumin and type one collagen with P. gingivalis cells or HmuY protein was examined using spectroscopic methods, SDS-PAGE and co-culturing P. gingivalis with human keratinocytes. We found that glycation increases the ability of P. gingivalis to acquire heme from hemoglobin, mostly due to heme sequestration by the HmuY hemophore-like protein. We also found an increase in biofilm formation on glycated collagen-coated abiotic surfaces. We conclude that glycation might promote the virulence of P. gingivalis by making heme more available from hemoglobin and facili-tating bacterial biofilm formation, thus increasing P. gingivalis pathogenic potential in vivo.

Item Type:	Article
Uncontrolled Keywords:	periodontitis, diabetes, glycation, Porphyromonas gingivalis, heme, HmuY, hemoglobin, albumin, collagen, pathogenesis
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	15 Nov 2021 11:27
Last Modified:	16 Jun 2026 10:17
DOI:	10.3390/ijms222112084
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3143193
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