3D localized lactate detection in muscle tissue using double‐quantum filtered 1 H MRS with adiabatic refocusing pulses at 7 T

Niess, Fabian, Roat, Sigrun, Bogner, Wolfgang, Krššák, Martin, Kemp, Graham J ORCID: 0000-0002-8324-9666, Schmid, Albrecht I, Trattnig, Siegfried, Moser, Ewald, Zaitsev, Maxim and Meyerspeer, Martin (2021) 3D localized lactate detection in muscle tissue using double‐quantum filtered 1 H MRS with adiabatic refocusing pulses at 7 T. Magnetic Resonance in Medicine, 87 (3). pp. 1174-1183. ISSN 0740-3194, 1522-2594

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Abstract

<h4>Purpose</h4>Lactate is a key metabolite in skeletal muscle and whole-body physiology. Its MR visibility in muscle is affected by overlapping lipid signals and fiber orientation. Double-quantum filtered (DQF) ¹ H MRS selectively detects lactate at 1.3 ppm, but at ultra-high field the efficiency of slice-selective 3D-localization with conventional RF pulses is limited by bandwidth. This novel 3D-localized ¹ H DQF MRS sequence uses adiabatic refocusing pulses to unambiguously detect lactate in skeletal muscle at 7 T.<h4>Methods</h4>Lactate double-quantum coherences were 3D-localized using slice-selective Shinnar-Le Roux optimized excitation and adiabatic refocusing pulses (similar to semi-LASER). DQF MR spectra were acquired at 7 T from lactate phantoms, meat specimens with injected lactate (exploring multiple TEs and fiber orientations), and human gastrocnemius in vivo during and after exercise (without cuff ischemia).<h4>Results</h4>Lactate was readily detected, achieving the full potential of 50% signal with a DQF, in solution. The effects of fiber orientation and TE on the lactate doublet (peak splitting, amplitude, and phase) were in good agreement with theory and literature. Exercise-induced lactate accumulation was detected with 30 s time resolution.<h4>Conclusion</h4>This novel 3D-localized ¹ H DQF MRS sequence can dynamically detect glycolytically generated lactate in muscle during exercise and recovery at 7 T.

Item Type:	Article
Uncontrolled Keywords:	double-quantum filter, lactate, localized 1H MRS, skeletal muscle exercise, ultra-high field
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	17 Nov 2021 09:19
Last Modified:	06 Dec 2024 19:49
DOI:	10.1002/mrm.29061
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3143311