Immunohistochemical expression and prognostic significance of MAGE-A in canine oral malignant melanoma

Guillen, Alexandra, Stiborova, Katerina, Ressel, Lorenzo ORCID: 0000-0002-6614-1223, Blackwood, Laura, Finotello, Riccardo ORCID: 0000-0002-1932-211X, Amores-Fuster, Isabel, Jama, Nimo and Killick, David ORCID: 0000-0002-8787-7651 (2021) Immunohistochemical expression and prognostic significance of MAGE-A in canine oral malignant melanoma. RESEARCH IN VETERINARY SCIENCE, 137. pp. 226-234.

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Abstract

Canine oral malignant melanoma (COMM) is considered a chemo-resistant cancer with a poor long-term prognosis. The melanoma-associated antigen A (MAGE-A) genes, which belong to the cancer-testis antigen family, are expressed in several different canine cancers but not in normal somatic tissue. This study evaluates the expression of MAGE-A proteins and their prognostic role in COMM. The study was conducted in 2 parts. During the first part, biopsies from oral malignant melanomas from 43 dogs were examined and immunohistochemically assessed for expression of MAGE-A proteins. For the second part, the association between MAGE-A expression and outcome was assessed using follow-up data which was available for 20 dogs whose primary tumour had been controlled with surgery +/- radiation therapy. MAGE-A proteins were expressed in 88.4% (38/43) of oral malignant melanomas and had a predominantly cytoplasmic expression pattern. Immunopositivity was observed in more than 50% of the cells in 21 dogs (48.8%). Immunostaining intensity was classified as weak, moderate and intense in 16 (37%), 16 (37%) and 6 (14%) cases, respectively. No staining for MAGE-A was seen in 5 dogs (11%). Dogs whose COMM had weak MAGE-A staining intensity had a median survival time (MST) of 320 days while this was 129 days for dogs with moderate and intense immunostaining (p = 0.161). Dogs whose COMM had >50% of positive staining neoplastic cells had an MST of 141 days and dogs with a staining <50% had an MST of 320 days (p = 0.164). MAGE-A expression did not influence survival in our cohort.

Item Type:	Article
Uncontrolled Keywords:	Immunohistochemistry, MAGE-A, Melanoma, Cancer testis antigens
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	29 Nov 2021 11:00
Last Modified:	18 Jan 2023 21:23
DOI:	10.1016/j.rvsc.2021.05.009
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3144153