Amoebicidal Activity of Poly-Epsilon-Lysine Functionalized Hydrogels

Kennedy, Stephnie M, Deshpande, Pallavi ORCID: 0000-0001-9714-9917, Gallagher, Andrew G, Horsburgh, Malcolm J ORCID: 0000-0002-3806-0039, Allison, Heather E ORCID: 0000-0003-0017-7992, Kaye, Stephen B ORCID: 0000-0003-0390-0592, Wellings, Donald A and Williams, Rachel L ORCID: 0000-0002-1954-0256
(2022) Amoebicidal Activity of Poly-Epsilon-Lysine Functionalized Hydrogels. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 63 (1). 11-.

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<h4>Purpose</h4>To determine the amoebicidal activity of functionalized poly-epsilon-lysine hydrogels (pɛK+) against Acanthamoeba castellanii.<h4>Methods</h4>A. castellanii trophozoites and cysts were grown in the presence of pɛK solution (0-2.17 mM), pɛK or pɛK+ hydrogels, or commercial hydrogel contact lens (CL) for 24 hours or 7 days in PBS or Peptone-Yeast-Glucose (PYG) media (nutrient-deplete or nutrient-replete cultures, respectively). Toxicity was determined using propidium iodide and imaged using fluorescence microscopy. Ex vivo porcine corneas were inoculated with A. castellanii trophozoites ± pɛK, pɛK+ hydrogels or commercial hydrogel CL for 7 days. Corneal infection was assessed by periodic acid-Schiff staining and histologic analysis. Regrowth of A. castellanii from hydrogel lenses and corneal discs at 7 days was assessed using microscopy and enumeration.<h4>Results</h4>The toxicity of pɛK+ hydrogels resulted in the death of 98.52% or 83.31% of the trophozoites at 24 hours or 7 days, respectively. The toxicity of pɛK+ hydrogels resulted in the death of 70.59% or 82.32% of the cysts in PBS at 24 hours or 7 days, respectively. Cysts exposed to pɛK+ hydrogels in PYG medium resulted in 75.37% and 87.14% death at 24 hours and 7 days. Ex vivo corneas infected with trophozoites and incubated with pɛK+ hydrogels showed the absence of A. castellanii in the stroma, with no regrowth from corneas or pɛK+ hydrogel, compared with infected-only corneas and those incubated in presence of commercial hydrogel CL.<h4>Conclusions</h4>pɛK+ hydrogels demonstrated pronounced amoebicidal and cysticidal activity against A. castellanii. pɛK+ hydrogels have the potential for use as CLs that could minimize the risk of CL-associated Acanthamoeba keratitis.

Item Type: Article
Uncontrolled Keywords: amoebicidal, hydrogels, antitrophozoite, anticyst
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 14 Dec 2021 09:04
Last Modified: 18 Jan 2023 21:20
DOI: 10.1167/iovs.63.1.11
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