Image fusion of IR and optical microscopy for mapping of biomolecules in tissue

Al Jedani, Safaa, Whitley, Conor A, Ellis, Barnaby G, Triantafyllou, Asterios, Smith, Caroline I ORCID: 0000-0001-6878-0697, Gunning, Philip J, Gardner, Peter, Risk, Janet M ORCID: 0000-0002-8770-7783, Weightman, Peter ORCID: 0000-0002-0907-3930 and Barrett, Steve D ORCID: 0000-0003-2960-3334 (2021) Image fusion of IR and optical microscopy for mapping of biomolecules in tissue. ANALYST, 146 (19). pp. 5848-5854. ISSN 0003-2654, 1364-5528

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Official URL: https://doi.org/10.1039/d1an01161h

Abstract

It is shown that a pixel-level image fusion technique can produce images that combine the spatial resolution of optical microscopy images of haematoxylin and eosin (H&E) stained tissue with the chemical information in Fourier transform infrared (FTIR) images. The fused images show minimal distortion and the higher spatial resolution of the H&E images overcomes the diffraction limit on the spatial resolution of the FTIR images. A consideration of the FTIR spectra of nucleic acids and collagen can explain the changes in contrast between non-cancerous oral epithelium and underlying stroma within fused images formed by combining an H&E stain of oral tissue with FTIR images of the tissue obtained at a number of wavenumbers.

Item Type:	Article
Uncontrolled Keywords:	Connective Tissue, Collagen, Microscopy, Spectroscopy, Fourier Transform Infrared, Fourier Analysis
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Dentistry Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology Faculty of Science and Engineering > School of Physical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	15 Dec 2021 16:11
Last Modified:	07 Dec 2024 23:12
DOI:	10.1039/d1an01161h
Open Access URL:	https://pubs.rsc.org/en/content/articlelanding/202...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3145437