High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways



Ortega, Eduardo, Nicholls, Daniel, Browning, Nigel D and de Jonge, Niels
(2021) High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways. SCIENTIFIC REPORTS, 11 (1). 22722-.

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Abstract

Scanning transmission electron microscopy (STEM) provides structural analysis with sub-angstrom resolution. But the pixel-by-pixel scanning process is a limiting factor in acquiring high-speed data. Different strategies have been implemented to increase scanning speeds while at the same time minimizing beam damage via optimizing the scanning strategy. Here, we achieve the highest possible scanning speed by eliminating the image acquisition dead time induced by the beam flyback time combined with reducing the amount of scanning pixels via sparse imaging. A calibration procedure was developed to compensate for the hysteresis of the magnetic scan coils. A combination of sparse and serpentine scanning routines was tested for a crystalline thin film, gold nanoparticles, and in an in-situ liquid phase STEM experiment. Frame rates of 92, 23 and 5.8 s<sup>-1</sup> were achieved for images of a width of 128, 256, and 512 pixels, respectively. The methods described here can be applied to single-particle tracking and analysis of radiation sensitive materials.

Item Type: Article
Uncontrolled Keywords: 40 Engineering, 51 Physical Sciences, 5104 Condensed Matter Physics, Bioengineering
Divisions: Faculty of Science and Engineering > School of Engineering
Depositing User: Symplectic Admin
Date Deposited: 20 Dec 2021 09:07
Last Modified: 21 Jun 2024 08:49
DOI: 10.1038/s41598-021-02052-1
Open Access URL: https://www.nature.com/articles/s41598-021-02052-1
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3145669