Meckiff, Benjamin J, Ramírez-Suástegui, Ciro, Fajardo, Vicente, Chee, Serena J, Kusnadi, Anthony, Simon, Hayley, Grifoni, Alba, Pelosi, Emanuela, Weiskopf, Daniela, Sette, Alessandro et al (show 4 more authors)
(2020)
Single-cell transcriptomic analysis of SARS-CoV-2 reactive CD4 + T cells.
bioRxiv, 1 (06-23).
2020.06.12.148916-.
Abstract
The contribution of CD4 + T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present large-scale single-cell transcriptomic analysis of viral antigen-reactive CD4 + T cells from 32 COVID-19 patients. In patients with severe disease compared to mild disease, we found increased proportions of cytotoxic follicular helper (T FH ) cells and cytotoxic T helper cells (CD4-CTLs) responding to SARS-CoV-2, and reduced proportion of SARS-CoV-2 reactive regulatory T cells. Importantly, the CD4-CTLs were highly enriched for the expression of transcripts encoding chemokines that are involved in the recruitment of myeloid cells and dendritic cells to the sites of viral infection. Polyfunctional T helper (T H )1 cells and T H 17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4 + T cells compared to influenza-reactive CD4 + T cells. Together, our analyses provide so far unprecedented insights into the gene expression patterns of SARS-CoV-2 reactive CD4 + T cells in distinct disease severities.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Emerging Infectious Diseases, Prevention, Infectious Diseases, Biodefense, Pneumonia & Influenza, Lung, Vaccine Related, 2.1 Biological and endogenous factors, 2 Aetiology, Inflammatory and immune system, Infection, 3 Good Health and Well Being |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 23 Dec 2021 08:02 |
| Last Modified: | 15 Mar 2024 20:25 |
| DOI: | 10.1101/2020.06.12.148916 |
| Open Access URL: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC75345... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3145886 |
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