Brown, Lottie, Byrne, Rachel L, Fraser, Alice, Owen, Sophie I, Cubas-Atienzar, Ana I, Williams, Christopher T, Kay, Grant A, Cuevas, Luis E, Fitchett, Joseph RA, Fletcher, Tom et al (show 9 more authors)
(2021)
Self-sampling of capillary blood for SARS-CoV-2 serology.
SCIENTIFIC REPORTS, 11 (1).
7754-.
ISSN 2045-2322, 2045-2322
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Text
Self-sampling of capillary blood for SARS-CoV-2 serology.pdf - Published version Download (914kB) | Preview |
Abstract
Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow tests. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty eight out of thirty nine participants were able to self-collect an adequate sample of capillary blood (≥ 50 µl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen's kappa coefficient of > 0.88 (near-perfect agreement, 95% CI 0.738-1.000). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.
| Item Type: | Article |
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| Uncontrolled Keywords: | Humans, Enzyme-Linked Immunosorbent Assay, Blood Specimen Collection, Adult, Middle Aged, Female, Male, Young Adult, Dried Blood Spot Testing, COVID-19, SARS-CoV-2, COVID-19 Serological Testing |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 01 Feb 2022 14:42 |
| Last Modified: | 06 Dec 2024 19:23 |
| DOI: | 10.1038/s41598-021-86008-5 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3147959 |
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