Skeletal muscle transcriptomics identifies common pathways in nerve crush injury and ageing



Staunton, CA ORCID: 0000-0003-0647-3063, Owen, ED, Hemmings, K ORCID: 0000-0001-5042-2226, Vasilaki, A ORCID: 0000-0002-5652-0895, McArdle, A, Barrett-Jolley, R ORCID: 0000-0003-0449-9972 and Jackson, MJ ORCID: 0000-0003-3683-8297
(2022) Skeletal muscle transcriptomics identifies common pathways in nerve crush injury and ageing. SKELETAL MUSCLE, 12 (1). 3-.

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Abstract

Motor unit remodelling involving repeated denervation and re-innervation occurs throughout life. The efficiency of this process declines with age contributing to neuromuscular deficits. This study investigated differentially expressed genes (DEG) in muscle following peroneal nerve crush to model motor unit remodelling in C57BL/6 J mice. Muscle RNA was isolated at 3 days post-crush, RNA libraries were generated using poly-A selection, sequenced and analysed using gene ontology and pathway tools. Three hundred thirty-four DEG were found in quiescent muscle from (26mnth) old compared with (4-6mnth) adult mice and these same DEG were present in muscle from adult mice following nerve crush. Peroneal crush induced 7133 DEG in muscles of adult and 699 DEG in muscles from old mice, although only one DEG (ZCCHC17) was found when directly comparing nerve-crushed muscles from old and adult mice. This analysis revealed key differences in muscle responses which may underlie the diminished ability of old mice to repair following nerve injury.

Item Type: Article
Uncontrolled Keywords: Skeletal muscle, Motor neuron, RNAseq, Transcriptomic, Ageing, Crush, Neurodegeneration
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User: Symplectic Admin
Date Deposited: 09 Feb 2022 10:49
Last Modified: 18 Jan 2023 21:13
DOI: 10.1186/s13395-021-00283-4
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3148583