Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications

Li, Man, Aveyard, Jenny, Doherty, Kyle G ORCID: 0000-0002-7616-1295, Deller, Robert C, Williams, Rachel L ORCID: 0000-0002-1954-0256, Kolegraff, Keli N, Kaye, Stephen B ORCID: 0000-0003-0390-0592 and D'Sa, Raechelle A ORCID: 0000-0003-2651-8783
(2022) Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications. ACS MATERIALS AU, 2 (2). pp. 190-203.

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Nonhealing and chronic wounds represent a major problem for the quality of life of patients and have cost implications for healthcare systems. The pathophysiological mechanisms that prevent wound healing are usually multifactorial and relate to patient overall health and nutrition, vascularity of the wound bed, and coexisting infection/colonization. Bacterial infections are one of the predominant issues that can stall a wound, causing it to become chronic. Successful wound healing often depends on weeks or months of antimicrobial therapy, but this is problematic given the rise in multidrug-resistant bacteria. As such, alternatives to antibiotics are desperately needed to aid the healing of chronic, and even acutely infected wounds. Nitric oxide (NO) kills bacteria through a variety of mechanisms, and thus, bacteria have shown no tendency to develop resistance to NO as a therapeutic agent and therefore can be a good alternative to antibiotic therapy. In this paper, we report on the development of NO-releasing electrospun membranes fabricated from polycaprolactone (PCL)/gelatin blends and optimized to reduce bacterial infection. The NO payload in the membranes was directly related to the number of amines (and hence the amount of gelatin) in the blend. Higher NO payloads corresponded with a higher degree of antimicrobial efficacy. No cytotoxicity was observed for electrospun membranes, and an <i>in vitro</i> wound closure assay demonstrated closure within 16 h. The results presented here clearly indicate that these NO-releasing electrospun membranes hold significant promise as wound dressings due to their antimicrobial activity and biocompatibility.

Item Type: Article
Uncontrolled Keywords: wound healing, nitric oxide, polycaprolactone, gelatin, antimicrobial activity, electrospinning
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Faculty of Science and Engineering > School of Engineering
Depositing User: Symplectic Admin
Date Deposited: 14 Feb 2022 16:51
Last Modified: 29 May 2023 20:45
DOI: 10.1021/acsmaterialsau.1c00056
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