Elsemman, Ibrahim E, Rodriguez Prado, Angelica, Grigaitis, Pranas, Garcia Albornoz, Manuel, Harman, Victoria 
ORCID: 0000-0002-0990-153X, Holman, Stephen W, van Heerden, Johan, Bruggeman, Frank J, Bisschops, Mark MM, Sonnenschein, Nikolaus et al (show 5 more authors)
(2022)
Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies.
Nature communications, 13 (1).
p. 801.
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Abstract
When conditions change, unicellular organisms rewire their metabolism to sustain cell maintenance and cellular growth. Such rewiring may be understood as resource re-allocation under cellular constraints. Eukaryal cells contain metabolically active organelles such as mitochondria, competing for cytosolic space and resources, and the nature of the relevant cellular constraints remain to be determined for such cells. Here, we present a comprehensive metabolic model of the yeast cell, based on its full metabolic reaction network extended with protein synthesis and degradation reactions. The model predicts metabolic fluxes and corresponding protein expression by constraining compartment-specific protein pools and maximising growth rate. Comparing model predictions with quantitative experimental data suggests that under glucose limitation, a mitochondrial constraint limits growth at the onset of ethanol formation-known as the Crabtree effect. Under sugar excess, however, a constraint on total cytosolic volume dictates overflow metabolism. Our comprehensive model thus identifies condition-dependent and compartment-specific constraints that can explain metabolic strategies and protein expression profiles from growth rate optimisation, providing a framework to understand metabolic adaptation in eukaryal cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Mitochondria, Saccharomyces cerevisiae, Yeasts, Glucose, Saccharomyces cerevisiae Proteins, Proteome, Proteomics, Gene Expression Regulation, Fungal, Fermentation, Metabolic Networks and Pathways |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 21 Feb 2022 08:52 |
| Last Modified: | 18 Jan 2023 21:12 |
| DOI: | 10.1038/s41467-022-28467-6 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3149305 |
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