Jose Vivero, Rafael, Alfonso Castaneda-Monsalve, Victor, Roberto Romero, Luis, Hurst, Gregory D 
ORCID: 0000-0002-7163-7784, Cadavid-Restrepo, Gloria and Ximena Moreno-Herrera, Claudia
(2021)
Gut Microbiota Dynamics in Natural Populations of Pintomyia evansi under Experimental Infection with Leishmania infantum.
MICROORGANISMS, 9 (6).
1214-.
Abstract
<i>Pintomyia evansi</i> is recognized by its vectorial competence in the transmission of parasites that cause fatal visceral leishmaniasis in rural and urban environments of the Caribbean coast of Colombia. The effect on and the variation of the gut microbiota in female <i>P. evansi</i> infected with <i>Leishmania infantum</i> were evaluated under experimental conditions using 16S rRNA Illumina MiSeq sequencing. In the coinfection assay with <i>L. infantum</i>, 96.8% of the midgut microbial population was composed mainly of Proteobacteria (71.0%), followed by Cyanobacteria (20.4%), Actinobacteria (2.7%), and Firmicutes (2.7%). In insect controls (uninfected with <i>L. infantum</i>) that were treated or not with antibiotics, <i>Ralstonia</i> was reported to have high relative abundance (55.1-64.8%), in contrast to guts with a high load of infection from <i>L. infantum</i> (23.4-35.9%). ASVs that moderately increased in guts infected with <i>Leishmania</i> were <i>Bacillus</i> and <i>Aeromonas</i>. Kruskal-Wallis nonparametric variance statistical inference showed statistically significant intergroup differences in the guts of <i>P. evansi</i> infected and uninfected with <i>L. infantum</i> (<i>p</i> < 0.05), suggesting that some individuals of the microbiota could induce or restrict <i>Leishmania</i> infection. This assay also showed a negative effect of the antibiotic treatment and <i>L. infantum</i> infection on the gut microbiota diversity. Endosymbionts, such as Microsporidia infections (<2%), were more often associated with guts without <i>Leishmania</i> infection, whereas <i>Arsenophonus</i> was only found in guts with a high load of <i>Leishmania</i> infection and treated with antibiotics. Finally, this is the first report that showed the potential role of intestinal microbiota in natural populations of <i>P. evansi</i> in susceptibility to <i>L. infantum</i> infection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Ralstonia, Arsenophonus, microsporidia, microbiota, Pintomyia evansi |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 28 Feb 2022 08:48 |
| Last Modified: | 24 Jan 2024 15:43 |
| DOI: | 10.3390/microorganisms9061214 |
| Open Access URL: | https://www.mdpi.com/2076-2607/9/6/1214 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3149747 |
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