Virome and Blood Meal-Associated Host Responses in <i>Ixodes persulcatus</i> Naturally Fed on Patients

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Li, Liang-Jing, Ning, Nian-Zhi, Zheng, Yuan-Chun, Chu, Yan-Li, Cui, Xiao-Ming, Zhang, Ming-Zhu, Guo, Wen-Bin, Wei, Ran, Liu, Hong-Bo, Sun, Yi et al (show 13 more authors) , Ye, Jin-Ling, Jiang, Bao-Gui, Yuan, Ting-Ting, Li, Jie, Bian, Cai, Bell-Sakyi, Lesley ORCID: 0000-0002-7305-0477, Wang, Hui, Jiang, Jia-Fu, Song, Ju-Liang, Cao, Wu-Chun, Tsan-Yuk Lam, Tommy, Ni, Xue-Bing and Jia, Na (2022) Virome and Blood Meal-Associated Host Responses in <i>Ixodes persulcatus</i> Naturally Fed on Patients. FRONTIERS IN MICROBIOLOGY, 12. 728996-.

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Abstract

The long-lasting co-evolution of ticks with pathogens results in mutual adaptation. Blood-feeding is one of the critical physiological behaviors that have been associated with the tick microbiome; however, most knowledge was gained through the study of laboratory-reared ticks. Here we detached <i>Ixodes persulcatus</i> ticks at different stages of blood-feeding from human patients and performed high-throughput transcriptomic analysis on them to identify their virome and genes differentially expressed between flat and fully fed ticks. We also traced bloodmeal sources of those ticks and identified bats and three other potential mammalian hosts, highlighting the public health significance. We found Jingmen tick virus and 13 putative new viruses belonging to 11 viral families, three of which even exhibited high genetic divergence from viruses previously reported in the same tick species from the same geographic region. Furthermore, differential expression analysis suggested a downregulation of antioxidant genes in the fully fed <i>I. persulcatus</i> ticks, which might be related to bloodmeal-related redox homeostasis. Our work highlights the significance of active surveillance of tick viromes and suggests a role of reactive oxygen species (ROS) in modulating changes in the microbiome during blood-feeding.

Item Type:	Article
Uncontrolled Keywords:	ticks, bloodmeal, virome, reactive oxygen species, patients, Jingmen tick virus
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	14 Mar 2022 17:06
Last Modified:	10 Oct 2023 23:26
DOI:	10.3389/fmicb.2021.728996
Open Access URL:	https://doi.org/10.3389/fmicb.2021.728996
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3150776