Multimodal imaging of synaptic vesicles with a single probe

An, Seong J, Stagi, Massimiliano ORCID: 0000-0002-5827-902X, Gould, Travis J, Wu, Yumei, Mlodzianoski, Michael, Rivera-Molina, Felix, Toomre, Derek, Strittmatter, Stephen M, De Camilli, Pietro, Bewersdorf, Joerg
et al (show 1 more authors) (2022) Multimodal imaging of synaptic vesicles with a single probe. Cell Reports Methods, 2 (4). p. 100199.

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A complete understanding of synaptic-vesicle recycling requires the use of multiple microscopy methods to obtain complementary information. However, many currently available probes are limited to a specific microscopy modality, which necessitates the use of multiple probes and labeling paradigms. Given the complexity of vesicle populations and recycling pathways, having new single-vesicle probes that could be used for multiple microscopy techniques would complement existing sets of tools for studying vesicle function. Here, we present a probe based on the membrane-binding C2 domain of cytosolic phospholipase A<sub>2</sub> (cPLA<sub>2</sub>) that fulfills this need. By conjugating the C2 domain with different detectable tags, we demonstrate that a single, modular probe can allow synaptic vesicles to be imaged at multiple levels of spatial and temporal resolution. Moreover, as a general endocytic marker, the C2 domain may also be used to study membrane recycling in many cell types.

Item Type: Article
Uncontrolled Keywords: Synaptic Vesicles, Multimodal Imaging
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 27 Apr 2022 15:14
Last Modified: 28 Sep 2023 18:22
DOI: 10.1016/j.crmeth.2022.100199
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