Azzolini, Claudio, Donati, Simone, Micheloni, Giovanni, Moretti, Vittoria, Valli, Roberto, Acquati, Francesco, Costantino, Lucy, Ferrara, Fulvio, Borroni, Davide 
ORCID: 0000-0001-6952-5647, Premi, Elias et al (show 3 more authors)
(2021)
Expression of <i>Otx</i> Genes in Muller Cells Using an <i>In Vitro</i> Experimental Model of Retinal Hypoxia.
JOURNAL OF OPHTHALMOLOGY, 2021.
6265553-.
Abstract
<h4>Introduction</h4>Müller glial cells typically activate to react to hypoxic tissue damage in several retinal diseases. We evaluated the <i>in vitro</i> response to a hypoxia-mimicking stimulus on the expression of a set of genes, known to contribute to eye morphogenesis and cell differentiation.<h4>Materials and methods</h4>A MIO-M1 Müller cell line was cultured in a hypoxia-mimicking environment by the addition of cobalt chloride to the culture medium, followed by a recovery time in which we mimic restoration from the hypoxic insult. The HIF-1<i>α</i> protein and VEGF-A gene expression were quantified to verify the induction of a hypoxia-like state.<h4>Results</h4>Among the genes under study, we did not observe any difference in the expression levels of <i>Otx1</i> and <i>Otx2</i> during treatment; conversely, <i>Otx1</i> was overexpressed during recovery steps. The VEGF-A gene was strongly upregulated at both the CoCl<sub>2</sub> and recovery time points. The transactivated isoform (TA) of the <i>TP73</i> gene showed an overexpression in long-term exposure to the hypoxic stimulus with a further increase after recovery. <i>Discussion</i>. Our molecular analysis is able to describe the activation of a set of genes, never before described, that can drive the response to a hypoxia-like status. The improved comprehension of these cellular events will be useful for designing new therapeutical approaches for retinal pathologies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Eye Disease and Disorders of Vision, Genetics, Neurosciences, Biotechnology, 5 Development of treatments and therapeutic interventions, 5.2 Cellular and gene therapies, 1.1 Normal biological development and functioning, 2.1 Biological and endogenous factors, 2 Aetiology, 1 Underpinning research, Eye |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 16 May 2022 12:43 |
| Last Modified: | 17 Mar 2024 13:18 |
| DOI: | 10.1155/2021/6265553 |
| Open Access URL: | https://www.hindawi.com/journals/joph/2021/6265553... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3154903 |
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