Su, Dunhao, Li, Yong, Yates, Edwin A 
ORCID: 0000-0001-9365-5433, Skidmore, Mark A, Lima, Marcelo A and Fernig, David G ORCID: 0000-0003-4875-4293
(2022)
Analysis of protein-heparin interactions using a portable SPR instrument.
PeerJ Analytical Chemistry, 4.
e15-e15.
ISSN 2691-6630, 2691-6630
Abstract
<jats:p>Optical biosensors such as those based on surface plasmon resonance (SPR) are a key analytical tool for understanding biomolecular interactions and function as well as the quantitative analysis of analytes in a wide variety of settings. The advent of portable SPR instruments enables analyses in the field. A critical step in method development is the passivation and functionalisation of the sensor surface. We describe the assembly of a surface of thiolated oleyl ethylene glycol/biotin oleyl ethylene glycol and its functionalisation with streptavidin and reducing end biotinylated heparin for a portable SPR instrument. Such surfaces can be batch prepared and stored. Two examples of the analysis of heparin-binding proteins are presented. The binding of fibroblast growth factor 2 and competition for the binding of a heparan sulfate sulfotransferase by a library of selectively modified heparins and suramin, which identify the selectivity of the enzyme for sulfated structures in the polysaccharide and demonstrate suramin as a competitor for the enzyme’s sugar acceptor site. Heparin functionalised surfaces should have a wide applicability, since this polysaccharide is a close structural analogue of the host cell surface polysaccharide, heparan sulfate, a receptor for many endogenous proteins and viruses.</jats:p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 3101 Biochemistry and Cell Biology, 34 Chemical Sciences, 31 Biological Sciences, Biotechnology, Generic health relevance |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 18 May 2022 09:09 |
| Last Modified: | 07 Dec 2024 22:23 |
| DOI: | 10.7717/peerj-achem.15 |
| Open Access URL: | https://peerj.com/articles/achem-15/# |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3155023 |
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