Govaert, Marlies, Smet, Cindy, Acquah, Cyril, Walsh, James L 
ORCID: 0000-0002-6318-0892 and Van Impe, Jan FM
(2022)
Behavior of the Surviving Population of Listeria monocytogenes and Salmonella Typhimurium Biofilms Following a Direct Helium-Based Cold Atmospheric Plasma Treatment.
FRONTIERS IN MICROBIOLOGY, 13.
831434-.
|
Text
Behavior of the Surviving Population of iListeria monocytogenesi and iSalmonellai Typhimurium Biofilms Following a Direct He.pdf - Published version Download (2MB) | Preview |
Abstract
Although the Cold Atmospheric Plasma (CAP) technology proved promising for inactivation of biofilms present on abiotic food contact surfaces, more research is required to examine the behavior of the CAP surviving biofilm-associated cells. It was therefore examined whether (i) CAP treated (<i>Listeria monocytogenes</i> and <i>Salmonella</i> Typhimurium) biofilm-associated cells were able to further colonize the already established biofilms during a subsequent incubation period and (ii) isolates of the surviving population became less susceptible toward CAP when the number of biofilm development-CAP treatment cycles increased. For this purpose, a direct treatment was applied using a helium-based Dielectric Barrier Discharge electrode configuration. Results indicated that the surviving population was able to further colonize the already established biofilms, since the cell density of the CAP treated + incubated biofilms equaled the initial density of the untreated biofilms. For the <i>L. monocytogenes</i> biofilms, also the total biomass proved to further increase, which might result in an even further increased resistance. The susceptibility of the biofilm-associated cells proved to be influenced by the specific number of CAP treatment cycles, which might potentially result in an overestimation of the CAP treatment efficacy and, consequently, an increased risk of food contamination.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | biofilms, cold atmospheric plasma, Listeria monocytogenes, regrowth, resistance and susceptibility, Salmonella Typhimurium, surviving population |
| Divisions: | Faculty of Science and Engineering > School of Electrical Engineering, Electronics and Computer Science |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 18 May 2022 09:42 |
| Last Modified: | 18 Jan 2023 21:01 |
| DOI: | 10.3389/fmicb.2022.831434 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3155029 |
Dimensions
Dimensions
