van der Hoek, Steven A, Rusnak, Matej, Jacobsen, Irene Hjorth, Martinez, Jose L, Kell, Douglas B ORCID: 0000-0001-5838-7963 and Borodina, Irina
(2021)
Engineering ergothioneine production in Yarrowia lipolytica.
FEBS LETTERS, 596 (10).
pp. 1356-1364.
Abstract
Ergothioneine is a naturally occurring antioxidant that has shown potential in ameliorating neurodegenerative and cardiovascular diseases. In this study, we investigated the potential of the Crabtree-negative, oleaginous yeast Yarrowia lipolytica as an alternative host for ergothioneine production. We expressed the biosynthetic enzymes EGT1 from Neurospora crassa and EGT2 from Claviceps purpurea to obtain 158 mg·L<sup>-1</sup> of ergothioneine in small-scale cultivation, with an additional copy of each gene improving the titer to 205 mg·L<sup>-1</sup> . The effect of phosphate limitation on ergothioneine production was studied, and finally, a phosphate-limited fed-batch fermentation in 1 L bioreactors yielded 1.63 ± 0.04 g·L<sup>-1</sup> ergothioneine in 220 h, corresponding to an overall volumetric productivity of 7.41 mg·L<sup>-1</sup> ·h<sup>-1</sup> , showing that Y. lipolytica is a promising host for ergothioneine production.
Item Type: | Article |
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Uncontrolled Keywords: | ergothioneine, metabolic engineering, nutraceutical, phosphate-limitation, Yarrowia lipolytica |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
Depositing User: | Symplectic Admin |
Date Deposited: | 18 May 2022 09:22 |
Last Modified: | 18 Jan 2023 21:01 |
DOI: | 10.1002/1873-3468.14239 |
Open Access URL: | https://doi.org/10.1002/1873-3468.14239 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3155031 |