Vascular Lakes in Uveal Melanoma and Their Association With Outcome.

Jones, Hayley, Kalirai, Helen ORCID: 0000-0002-4440-2576, Taktak, Azzam, Chen, Ke ORCID: 0000-0002-6093-6623 and Coupland, Sarah E ORCID: 0000-0002-1464-2069
(2022) Vascular Lakes in Uveal Melanoma and Their Association With Outcome. Translational vision science & technology, 11 (3). p. 32.

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<h4>Purpose</h4>Prognostic predictors in uveal melanoma (UM) consist of clinical, histomorphologic, and genetic features. Vascular lakes (VLs) are immature blood vessels within UM with unknown significance for metastatic risk.<h4>Methods</h4>A clinically well-phenotyped cohort of 136 hematoxylin and eosin-stained slides of UM enucleation specimens were retrospectively analyzed on scanned whole-slide images. These were annotated for VL in QuPath, assessing VL number and area. Using SPSS (V27.0), the Mann-Whitney U test and Cox regression were applied to evaluate whether there was any correlation between VL number and area within the tumor (VL-TA) compared with other prognostic parameters and patient survival times.<h4>Results</h4>UMs with monosomy 3 (M3) have significant differences in their VL numbers (P = 0.008) and VL-TA ratios (P = 0.002) compared with disomy 3-UM. Nuclear BAP1-negative (nBAP1-) UMs have significant differences in their VL-TA ratio (P = 0.002) compared to nBAP1+ UMs. Survival times of patients with UM with epithelioid-celled tumors varied depending on their VL-TA ratio (P = 0.057). Similarly, in M3-UM, significant differences in survival (P = 0.009) were seen in patients, depending on VL number. Finally, patients with UM with shorter overall survival showed significant differences in their tumor VL-TA ratios (P = 0.043) and the number of VLs present (P = 0.002) than patients with UM who had longer survival.<h4>Conclusions</h4>Our pilot data suggest that VL-TA is an additional poor prognostic parameter in UM.<h4>Translational relevance</h4>Digital analysis of UM can be easily performed to assess various prognostic parameters. Our pilot study demonstrates that UM-VL could be combined with other parameters to determine metastatic risk of patients with UM.

Item Type: Article
Uncontrolled Keywords: uveal melanoma, molecular genetics, prognostication, BAP1
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 25 May 2022 09:09
Last Modified: 28 Jun 2023 22:29
DOI: 10.1167/tvst.11.3.32
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