Quantitative proteomic analysis of bronchoalveolar lavage fluid in West Highland white terriers with canine idiopathic pulmonary fibrosis



Maher, Rosemary E ORCID: 0000-0002-2137-6619, Maatta, Merita, Beynon, Robert J ORCID: 0000-0003-0857-495X, Laurila, Henna P, McNamara, Paul S ORCID: 0000-0002-7055-6034 and Rajamaki, Minna M
(2022) Quantitative proteomic analysis of bronchoalveolar lavage fluid in West Highland white terriers with canine idiopathic pulmonary fibrosis. BMC VETERINARY RESEARCH, 18 (1). 121-.

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Abstract

<h4>Background</h4>Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, progressive, interstitial fibrosing lung disease, manifesting as cough, exercise intolerance and ultimately, dyspnea and respiratory failure. It mainly affects West Highland white terriers (WHWTs), lacks curable treatment and has a poor prognosis. Aspiration of gastroesophageal refluxate may play a role in the development of CIPF. In the first part of this study, we completed label-free quantitative proteomic analysis of bronchoalveolar lavage fluid (BALF) from CIPF and healthy WHWTs. In the second part, we evaluated potential protein markers of reflux aspiration from canine gastric juice and vomitus and whether these were present in BALF from the two groups.<h4>Results</h4>Across all BALF samples, 417 proteins were identified, and of these, 265 proteins were identified by two or more unique tryptic peptides. Using the 265 high confidence assignments, the quantitative proteome profiles were very similar in the two cohorts, but they could be readily resolved by principal component analysis on the basis of differential protein expression. Of the proteins that were differentially abundant in the two groups, several (including inflammatory and fibrotic markers) were elevated in CIPF, and a smaller, more diverse group of proteins were diminished in CIPF. No protein markers indicative of reflux aspiration were identified.<h4>Conclusions</h4>Label-free proteomics allowed discrimination between CIPF and healthy WHWTs, consistent with fibrotic process but did not provide clear evidence for gastrointestinal aspiration. The measurement of proteins may provide a proteomics signature of CIPF that could be used to evaluate treatment options.

Item Type: Article
Uncontrolled Keywords: Bronchoalveolar lavage fluid, Canine, CIPF, Dog, Lung, Proteomics, Reflux aspiration, Reflux aspiration, West Highland white terrier
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 31 May 2022 16:01
Last Modified: 18 Jan 2023 21:00
DOI: 10.1186/s12917-022-03202-x
Open Access URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC89661...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3155756

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