Kathirvel, Kandasamy, Karen, Lester, Haribalaganesh, Ravinarayanan, Krishnadas, Ramasamy, Muthukkaruppan, Veerappan, Lane, Brian, Simpson, David A, Goljanek-Whysall, Kasia 
ORCID: 0000-0001-8166-8800, Sheridan, Carl ORCID: 0000-0003-0100-9587, Bharanidharan, Devarajan et al (show 2 more authors)
(2022)
Short and long-term effect of dexamethasone on the transcriptome profile of primary human trabecular meshwork cells in vitro
SCIENTIFIC REPORTS, 12 (1).
8299-.
ISSN 2045-2322, 2045-2322
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Text
Short and long-term effect of dexamethasone on the transcriptome profile of primary human trabecular meshwork cells in vitro.pdf - Published version Download (1MB) | Preview |
Abstract
In the quest of identifying newer molecular targets for the management of glucocorticoid-induced ocular hypertension (GC-OHT) and glaucoma (GCG), several microarray studies have attempted to investigate the genome-wide transcriptome profiling of primary human trabecular meshwork (TM) cells in response to dexamethasone (DEX). However, no studies are reported so far to demonstrate the temporal changes in the expression of genes in the cultured human TM cells in response to DEX treatment. Therefore, in the present study, the time-dependent changes in the genome-wide expression of genes in primary human TM cells after short (16 hours: 16 h) and long exposure (7 days: 7 d) of DEX was investigated using RNA sequencing. There were 199 (118 up-regulated; 81 down-regulated) and 525 (119 up-regulated; 406 down-regulated) DEGs in 16 h and 7 d treatment groups respectively. The unique genes identified in 16 h and 7 d treatment groups were 152 and 478 respectively. This study found a distinct gene signature and pathways between two treatment regimes. Longer exposure of DEX treatment showed a dys-regulation of Wnt and Rap1 signaling and so highlighted potential therapeutic targets for pharmacological management of GC-OHT/glaucoma.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Trabecular Meshwork, Cells, Cultured, Humans, Glaucoma, Dexamethasone, Glucocorticoids, Transcriptome |
| Divisions: | Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 07 Jun 2022 13:12 |
| Last Modified: | 16 Jun 2026 11:19 |
| DOI: | 10.1038/s41598-022-12443-7 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3156009 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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