Scaffold-Free Strategy Using a PEG-Dextran Aqueous Two-Phase-System for Corneal Tissue Repair.

Hung, Lap Tak, Poon, Stephanie Hiu Ling, Yan, Wing Huen, Lace, Rebecca, Zhou, Liangyu, Wong, Jasper Ka Wai, Williams, Rachel L ORCID: 0000-0002-1954-0256, Shih, Kendrick Co, Shum, Ho Cheung and Chan, Yau Kei (2022) Scaffold-Free Strategy Using a PEG-Dextran Aqueous Two-Phase-System for Corneal Tissue Repair. ACS biomaterials science & engineering, 8 (5). pp. 1987-1999. ISSN 2373-9878, 2373-9878

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Abstract

Forming thin tissue constructs with minimal extracellular matrix surrounding them is important for tissue engineering applications. Here, we explore and optimize a strategy that enables rapid fabrication of scaffold-free corneal tissue constructs using the liquid-liquid interface of an aqueous two-phase system (ATPS) that is based on biocompatible polymers, dextran and polyethylene glycol. Intact tissue-like constructs, made of corneal epithelial or endothelial cells, can be formed on the interface between the two liquid phases of ATPS within hours and subsequently collected simply by removing the liquid phases. The formed corneal cell constructs express essential physiological markers and have preserved viability and proliferative ability in vitro. The corneal epithelial cell constructs are also able to re-epithelialize the corneal epithelial wound in vitro. The results suggest the promise of our reported strategy in corneal repair.

Item Type:	Article
Uncontrolled Keywords:	tissue engineering, ophthalmology, scaffold free, wound healing, cornea
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	09 Jun 2022 08:04
Last Modified:	01 Mar 2026 00:17
DOI:	10.1021/acsbiomaterials.1c01500
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3156099
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