Exploiting the genome of <i>Thinopyrum elongatum</i> to expand the gene pool of hexaploid wheat

Baker, Lauren, Grewal, Surbhi, Yang, Cai-yun, Hubbart-Edwards, Stella, Scholefield, Duncan, Ashling, Stephen, Burridge, Amanda J, Przewieslik-Allen, Alexandra M, Wilkinson, Paul A ORCID: 0000-0002-8836-5439, King, Ian P
et al (show 1 more authors) (2020) Exploiting the genome of <i>Thinopyrum elongatum</i> to expand the gene pool of hexaploid wheat. THEORETICAL AND APPLIED GENETICS, 133 (7). pp. 2213-2226.

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<h4>Key message</h4>One hundred and thirty four introgressions from Thinopyrum elongatum have been transferred into a wheat background and were characterised using 263 SNP markers. Species within the genus Thinopyrum have been shown to carry genetic variation for a very wide range of traits including biotic and abiotic stresses and quality. Research has shown that one of the species within this genus, Th. elongatum, has a close relationship with the genomes of wheat making it a highly suitable candidate to expand the gene pool of wheat. Homoeologous recombination, in the absence of the Ph1 gene, has been exploited to transfer an estimated 134 introgressions from Th. elongatum into a hexaploid wheat background. The introgressions were detected and characterised using 263 single nucleotide polymorphism markers from a 35 K Axiom<sup>®</sup> Wheat-Relative Genotyping Array, spread across seven linkage groups and validated using genomic in situ hybridisation. The genetic map had a total length of 187.8 cM and the average chromosome length was 26.8 cM. Comparative analyses of the genetic map of Th. elongatum and the physical map of hexaploid wheat confirmed previous work that indicated good synteny at the macro-level, although Th. elongatum does not contain the 4A/5A/7B translocation found in wheat.

Item Type: Article
Uncontrolled Keywords: Chromosomes, Plant, Poaceae, Triticum, Genetic Markers, Chromosome Mapping, Physical Chromosome Mapping, Synteny, Gene Pool, Genotype, Phenotype, Ploidies, Polymorphism, Single Nucleotide, Genome, Plant, Genetic Linkage
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 10 Jun 2022 14:07
Last Modified: 06 Oct 2023 17:06
DOI: 10.1007/s00122-020-03591-3
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3156189