Preparation of oleylethylene glycol-sreptavidin surfaces for SPR v2


Fernig, Dave ORCID: 0000-0003-4875-4293 and Su, Dunhao ORCID: 0000-0002-9760-3419 (2021) Preparation of oleylethylene glycol-sreptavidin surfaces for SPR v2

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Abstract

<p>Surface plasmon resonance uses gold surfaces for sensing. Manufacturers provide a range of pre-functionalised surfaces, but these are often prone to non-specific binding problems. In other surface science sensing techniques a range of surface functionalisation approaches have been described. Here, the preparation of a self-assembled monolayer (SAM) of a thiolated oleyl ethylene glycol, incorporating a defined mole % of biotinylated ligands , on a gold surface is described. This allows the formation of a streptavidin layer on the SAM with control over the average surface coverage of streptavidin. Biotinylated ligands can then be immobilised on the streptavidin. Such surfaces have proved to be very resistant to non-specific binding and they are easily implemented on the sensor surfaces of commercial (surface plasmon resonance) SPR instruments. This is adapted from a published method: Migliorini, E. et al. Well-defined biomimetic surfaces to characterize glycosaminoglycan-mediated interactions on the molecular, supramolecular and cellular levels. Biomaterials (2014) doi:10.1016/j.biomaterials.2014.07.017.</p>

Item Type: Article
Divisions: Faculty of Health & Life Sciences
Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 05 Aug 2022 10:14
Last Modified: 06 Dec 2025 07:10
DOI: 10.17504/protocols.io.byv9pw96
Open Access URL: https://www.protocols.io/view/preparation-of-oleyl...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3160434
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