Assessment of creatinine concentration in whole blood spheroids using paper spray ionization–tandem mass spectrometry

Sham, Tung-Ting ORCID: 0000-0002-9648-4964, Badu-Tawiah, Abraham K, McWilliam, Stephen J ORCID: 0000-0002-0509-7425 and Maher, Simon
(2022) Assessment of creatinine concentration in whole blood spheroids using paper spray ionization–tandem mass spectrometry. Scientific Reports, 12 (1). 14308-.

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<jats:title>Abstract</jats:title><jats:p>Accurate quantification of blood creatinine is important to estimate the glomerular filtration rate. Existing techniques using liquid chromatography tandem mass spectrometry (LC–MS/MS) have a high accuracy and eliminate most interferences encountered in routine enzymatic and Jaffé methods. However, they require laborious and time-consuming sample treatment and data acquisition. The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC–MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization—tandem mass spectrometry (PSI–MS/MS). The method is validated using real human samples and compared with LC–MS/MS. PSI–MS/MS whole blood analysis exhibited a lower limit of quantification of 2.5 μg/mL, precision ≤ 6.3%, recovery in the range of 88–94% and excellent linearity (R<jats:sup>2</jats:sup> &gt; 0.99; 2.5—20 μg/mL) covering the normal range for creatinine levels. Creatinine levels were comparable to those measured by LC–MS/MS with small deviations of less than 0.3 μg/mL. This simple, fast and accurate microsampling technique for direct analysis of creatinine from whole blood shows promise for routine clinical screening and monitoring. This approach can be readily extended for other analytes of interest and, due to inherent advantages relating to cost, storability, speed, and simplicity, it can be especially advantageous for use in resource-limited settings.</jats:p>

Item Type: Article
Uncontrolled Keywords: Humans, Creatinine, Glomerular Filtration Rate, Chromatography, Liquid, Chromatography, High Pressure Liquid, Reproducibility of Results, Tandem Mass Spectrometry
Divisions: Faculty of Science and Engineering > School of Electrical Engineering, Electronics and Computer Science
Depositing User: Symplectic Admin
Date Deposited: 23 Aug 2022 07:12
Last Modified: 18 Jan 2023 20:47
DOI: 10.1038/s41598-022-18365-8
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