Arsenophonus apicola sp. nov., isolated from the honeybee Apis mellifera

Nadal-Jimenez, Pol ORCID: 0000-0002-9883-0920, Siozios, Stefanos ORCID: 0000-0002-1104-7061, Frost, Crystal L, Court, Rebecca, Chrostek, Ewa ORCID: 0000-0003-1825-372X, Drew, Georgia C, Evans, Jay D, Hawthorne, David J, Burritt, James B and Hurst, Gregory DD ORCID: 0000-0002-7163-7784
(2022) Arsenophonus apicola sp. nov., isolated from the honeybee Apis mellifera. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 72 (8).

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The genus <i>Arsenophonus</i> has been traditionally considered to comprise heritable bacterial symbionts of arthropods. Recent work has reported a microbe related to the type species <i>Arsenophonus nasoniae</i> as infecting the honey bee, <i>Apis mellifera</i>. The association was unusual for members of the genus in that the microbe-host interaction arose through environmental and social exposure rather than vertical transmission. In this study, we describe the <i>in vitro</i> culture of ArsBeeUS<sup>T</sup>, a strain of this microbe isolated from <i>A. mellifera</i> in the USA. The 16S rRNA sequence of the isolated strain indicates it falls within the genus <i>Arsenophonus</i>. Biolog analysis indicates the bacterium has a restricted range of nutrients that support growth. <i>In vivo</i> experiments demonstrate the strain proliferates rapidly on injection into <i>A. mellifera</i> hosts. We further report the closed genome sequence for the strain. The genome is 3.3 Mb and the G+C content is 37.6 mol%, which is smaller than <i>A. nasoniae</i> but larger than the genomes reported for non-culturable <i>Arsenophonus</i> symbionts. The genome is complex, with six extrachromosomal elements and 11 predicted intact phage elements, but notably less complex than <i>A. nasoniae</i>. Strain ArsBeeUS<sup>T</sup> is clearly distinct from the type species <i>A. nasoniae</i> on the basis of genome sequence, with 92 % average nucleotide identity. Based on our results, we propose <i>Arsenophonus apicola</i> sp. nov., with the type strain ArsBeeUS<sup>T</sup> (CECT 30499<sup>T</sup>=DSM113403<sup>T</sup>=LMG 32504<sup>T</sup>).

Item Type: Article
Uncontrolled Keywords: Arsenophonus, Apis mellifera, symbiosis
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 13 Sep 2022 14:06
Last Modified: 18 Jan 2023 20:45
DOI: 10.1099/ijsem.0.005469
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