Marine Synechococcus sp. Strain WH7803 Shows Specific Adaptative Responses to Assimilate Nanomolar Concentrations of Nitrate



Agustina Dominguez-Martin, Maria, Lopez-Lozano, Antonio, Melero-Rubio, Yesica, Gomez-Baena, Guadalupe ORCID: 0000-0003-3796-3874, Andres Jimenez-Estrada, Juan, Kukil, Kateryna, Diez, Jesus and Manuel Garcia-Fernandez, Jose
(2022) Marine Synechococcus sp. Strain WH7803 Shows Specific Adaptative Responses to Assimilate Nanomolar Concentrations of Nitrate. MICROBIOLOGY SPECTRUM, 10 (4). e0018722-.

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Abstract

Marine <i>Synechococcus</i>, together with <i>Prochlorococcus</i>, contribute to a significant proportion of the primary production on Earth. The spatial distribution of these two groups of marine picocyanobacteria depends on different factors such as nutrient availability and temperature. Some <i>Synechococcus</i> ecotypes thrive in mesotrophic and moderately oligotrophic waters, where they exploit both oxidized and reduced forms of nitrogen. Here, we present a comprehensive study, which includes transcriptomic and proteomic analyses of the response of <i>Synechococcus</i> sp. strain WH7803 to nanomolar concentrations of nitrate, compared to micromolar ammonium or nitrogen starvation. We found that <i>Synechococcus</i> has a specific response to a nanomolar nitrate concentration that differs from the response shown under nitrogen starvation or the presence of standard concentrations of either ammonium or nitrate. This fact suggests that the particular response to the uptake of nanomolar concentrations of nitrate could be an evolutionary advantage for marine <i>Synechococcus</i> against <i>Prochlorococcus</i> in the natural environment. <b>IMPORTANCE</b> Marine <i>Synechococcus</i> are a very abundant group of photosynthetic organisms on our planet. Previous studies have shown blooms of these organisms when nanomolar concentrations of nitrate become available. We have assessed the effect of nanomolar nitrate concentrations by studying the transcriptome and proteome of <i>Synechococcus</i> sp. WH7803, together with some physiological parameters. We found evidence that <i>Synechococcus</i> sp. strain WH7803 does sense and react to nanomolar concentrations of nitrate, suggesting the occurrence of specific adaptive mechanisms to allow their utilization. Thus, very low concentrations of nitrate in the ocean seem to be a significant nitrogen source for marine picocyanobacteria.

Item Type: Article
Uncontrolled Keywords: Synechococcus WH7803, nitrate, nitrogen metabolism, proteomics, transcriptomics
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 17 Oct 2022 14:33
Last Modified: 18 Jan 2023 20:36
DOI: 10.1128/spectrum.00187-22
Open Access URL: https://doi.org/10.1128/spectrum.00187-22
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3165547