Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min

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Rodriguez-Manzano, Jesus, Malpartida-Cardenas, Kenny, Moser, Nicolas, Pennisi, Ivana, Cavuto, Matthew, Miglietta, Luca, Moniri, Ahmad, Penn, Rebecca, Satta, Giovanni, Randell, Paul et al (show 5 more authors) , Davies, Frances, Bolt, Frances, Barclay, Wendy, Holmes, Alison ORCID: 0000-0001-5554-5743 and Georgiou, Pantelis (2021) Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min ACS CENTRAL SCIENCE, 7 (2). pp. 307-317. ISSN 2374-7943, 2374-7951

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Abstract

The COVID-19 pandemic is a global health emergency characterized by the high rate of transmission and ongoing increase of cases globally. Rapid point-of-care (PoC) diagnostics to detect the causative virus, SARS-CoV-2, are urgently needed to identify and isolate patients, contain its spread and guide clinical management. In this work, we report the development of a rapid PoC diagnostic test (<20 min) based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and semiconductor technology for the detection of SARS-CoV-2 from extracted RNA samples. The developed LAMP assay was tested on a real-time benchtop instrument (RT-qLAMP) showing a lower limit of detection of 10 RNA copies per reaction. It was validated against extracted RNA from 183 clinical samples including 127 positive samples (screened by the CDC RT-qPCR assay). Results showed 91% sensitivity and 100% specificity when compared to RT-qPCR and average positive detection times of 15.45 ± 4.43 min. For validating the incorporation of the RT-LAMP assay onto our PoC platform (RT-eLAMP), a subset of samples was tested (<i>n</i> = 52), showing average detection times of 12.68 ± 2.56 min for positive samples (<i>n</i> = 34), demonstrating a comparable performance to a benchtop commercial instrument. Paired with a smartphone for results visualization and geolocalization, this portable diagnostic platform with secure cloud connectivity will enable real-time case identification and epidemiological surveillance.

Item Type:	Article
Uncontrolled Keywords:	34 Chemical Sciences, Bioengineering, Emerging Infectious Diseases, Coronaviruses, Infectious Diseases, Coronaviruses Diagnostics and Prognostics, Biotechnology, 4.1 Discovery and preclinical testing of markers and technologies, Infection
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	28 Oct 2022 14:30
Last Modified:	16 Jan 2026 10:36
DOI:	10.1021/acscentsci.0c01288
Open Access URL:	https://pubs.acs.org/doi/pdf/10.1021/acscentsci.0c...
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3165831
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