The Expression of Glutaminases and their Association with Clinicopathological Parameters in the Head and Neck Cancers

Basheer, Haneen A, Elsalem, Lina, Salem, Anwar, Tailor, Artysha, Hunter, Keith ORCID: 0000-0002-7873-0877 and Afarinkia, Kamyar
(2022) The Expression of Glutaminases and their Association with Clinicopathological Parameters in the Head and Neck Cancers. CURRENT CANCER DRUG TARGETS, 22 (2). pp. 169-179.

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<h4>Background</h4>The increased glutamine metabolism is a characteristic feature of cancer cells. The interconversion between glutamine and glutamate is catalyzed by two glutaminase isoforms, GLS1 and GLS2, which appear to have different roles in different types of cancer. We investigated for the first time the protein expression of GLS1 and GLS2, and their correlation with advanced clinicopathological parameters in head and neck cancers.<h4>Methods</h4>Consecutive slides from a tissue microarray comprised of 80 samples ranging from normal to metastatic were stained immunohistochemically for GLS1, GLS2, HIF-1α or CD147. Following analysis by two expert pathologists, we carried out a statistical analysis of the scores.<h4>Results</h4>GLS1 and GLS2 were found to be upregulated at the protein level in head and neck tumours compared to normal tissues, and this increased expression correlated positively (GLS1) and negatively (GLS2) with tumor grade, indicating a shift of expression between GLS enzyme isoforms based on tumor differentiation. Increased expression of GLS1 was associated with high CD147 expression, and elevated GLS2 expression was associated with both high CD147 and high HIF-1α expressions. The correlation of the GLS1 and GLS2 with HIF-1α or CD147 was strongly associated with more advanced clinicopathological parameters.<h4>Conclusion</h4>The increased expression of GLS1 and GLS2 may be explored as a new treatment for head and neck cancers.

Item Type: Article
Uncontrolled Keywords: Glutaminases, GLS1, GLS2, CD147, immunohistochemistry, head and neck cancers
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 22 Nov 2022 11:20
Last Modified: 15 Mar 2024 19:10
DOI: 10.2174/1568009622666211224111425
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