Chen, Taiyu, Riaz, Saba, Davey, Philip, Zhao, Ziyu, Sun, Yaqi, Dykes, Gregory, Zhou, Fei, Hartwell, James ORCID: 0000-0001-5000-223X, Lawson, Tracy, Nixon, Peter et al (show 2 more authors)
(2023)
Producing fast and active Rubisco in tobacco to enhance photosynthesis.
The Plant Cell, 35 (2).
pp. 795-807.
ISSN 1040-4651, 1532-298X
PDF
2022_10 Rubisco engineering plant_PC.pdf - Author Accepted Manuscript Download (1MB) | Preview |
Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) performs most of the carbon fixation on Earth. However, plant Rubisco is an intrinsically inefficient enzyme given its low carboxylation rate, representing a major limitation to photosynthesis. Replacing endogenous plant Rubisco with a faster Rubisco is anticipated to enhance crop photosynthesis and productivity. However, the requirement of chaperones for Rubisco expression and assembly has obstructed the efficient production of functional foreign Rubisco in chloroplasts. Here, we report the engineering of a Form 1A Rubisco from the proteobacterium Halothiobacillus neapolitanus in Escherichia coli and tobacco (Nicotiana tabacum) chloroplasts without any cognate chaperones. The native tobacco gene encoding Rubisco large subunit was genetically replaced with H. neapolitanus Rubisco (HnRubisco) large and small subunit genes. We show that HnRubisco subunits can form functional L8S8 hexadecamers in tobacco chloroplasts at high efficiency, accounting for ∼40% of the wild-type tobacco Rubisco content. The chloroplast-expressed HnRubisco displayed a ∼2-fold greater carboxylation rate and supported a similar autotrophic growth rate of transgenic plants to that of wild-type in air supplemented with 1% CO2. This study represents a step toward the engineering of a fast and highly active Rubisco in chloroplasts to improve crop photosynthesis and growth.
Item Type: | Article |
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Uncontrolled Keywords: | Chloroplasts, Plants, Genetically Modified, Carbon Dioxide, Ribulose-Bisphosphate Carboxylase, Photosynthesis, Nicotiana |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
Depositing User: | Symplectic Admin |
Date Deposited: | 13 Dec 2022 10:20 |
Last Modified: | 06 Dec 2024 20:04 |
DOI: | 10.1093/plcell/koac348 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3166626 |