Exploring the Healthy Eye Microbiota Niche in a Multicenter Study

Borroni, Davide ORCID: 0000-0001-6952-5647, Paytuvi-Gallart, Andreu, Sanseverino, Walter, Gomez-Huertas, Carmen, Bonci, Paola, Romano, Vito ORCID: 0000-0002-5148-7643, Giannaccare, Giuseppe, Rechichi, Miguel, Meduri, Alessandro, Oliverio, Giovanni William
et al (show 1 more authors) (2022) Exploring the Healthy Eye Microbiota Niche in a Multicenter Study. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (18). 10229-.

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<h4>Purpose</h4>This study aims to explore and characterize healthy eye microbiota.<h4>Methods</h4>Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end-repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02).<h4>Results</h4>Healthy eye microbiota is a low-diversity microbiome. The vast majority of the 137 analyzed samples were highly enriched with <i>Staphylococcus</i>, whereas only in a few of them, other genera such as <i>Bacillus, Pseudomonas,</i> and <i>Corynebacterium</i> predominate. We found an average of 88 genera with an average Shannon index of 0.65.<h4>Conclusion</h4>We identified nine different ECSTs. A better understanding of healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to promote health.

Item Type: Article
Uncontrolled Keywords: microbiome, healthy, metagenomics, eye, 16, OSDI
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Faculty of Health and Life Sciences > Clinical Directorate
Depositing User: Symplectic Admin
Date Deposited: 31 Jan 2023 10:39
Last Modified: 31 Jan 2023 10:39
DOI: 10.3390/ijms231810229
Open Access URL: https://doi.org/10.3390/ijms231810229
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3168000