Sagi-Kiss, Virag, Li, Yufeng, Carey, Matthew R, Grover, Sarah J, Siems, Karsten, Cirulli, Francesca, Berry, Alessandra, Musillo, Chiara, Wilson, Ian D 
ORCID: 0000-0002-8558-7394, Want, Elizabeth J et al (show 1 more authors)
(2022)
Ion-Pairing Chromatography and Amine Derivatization Provide Complementary Approaches for the Targeted LC-MS Analysis of the Polar Metabolome.
JOURNAL OF PROTEOME RESEARCH, 21 (6).
pp. 1428-1437.
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Abstract
Liquid chromatography coupled to mass spectrometry is a key metabolomics/metabonomics technology. Reversed-phase liquid chromatography (RPLC) is very widely used as a separation step, but typically has poor retention of highly polar metabolites. Here, we evaluated the combination of two alternative methods for improving retention of polar metabolites based on 6-aminoquinoloyl-<i>N</i>-hydroxysuccinidimyl carbamate derivatization for amine groups, and ion-pairing chromatography (IPC) using tributylamine as an ion-pairing agent to retain acids. We compared both of these methods to RPLC and also to each other, for targeted analysis using a triple-quadrupole mass spectrometer, applied to a library of ca. 500 polar metabolites. IPC and derivatization were complementary in terms of their coverage: combined, they improved the proportion of metabolites with good retention to 91%, compared to just 39% for RPLC alone. The combined method was assessed by analyzing a set of liver extracts from aged male and female mice that had been treated with the polyphenol compound ampelopsin. Not only were a number of significantly changed metabolites detected, but also it could be shown that there was a clear interaction between ampelopsin treatment and sex, in that the direction of metabolite change was opposite for males and females.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | metabolomics, metabonomics, ion-pairing, ampelopsin, healthy aging, UPLC-MS, NMR spectroscopy, statistical heterospectroscopy |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 08 Feb 2023 14:57 |
| Last Modified: | 08 Feb 2023 14:58 |
| DOI: | 10.1021/acs.jproteome.2c00030 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3168272 |
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