Synthetic engineering of a new biocatalyst encapsulating [NiFe]-hydrogenases for enhanced hydrogen production

Jiang, Qiuyao, Li, Tianpei, Yang, Jing, Aitchison, Catherine M, Huang, Jiafeng, Chen, Yu, Huang, Fang, Wang, Qiang, Cooper, Andrew I ORCID: 0000-0003-0201-1021 and Liu, Lu-Ning ORCID: 0000-0002-8884-4819
(2023) Synthetic engineering of a new biocatalyst encapsulating [NiFe]-hydrogenases for enhanced hydrogen production. JOURNAL OF MATERIALS CHEMISTRY B, 11 (12). pp. 2684-2692.

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Hydrogenases are microbial metalloenzymes capable of catalyzing the reversible interconversion between molecular hydrogen and protons with high efficiency, and have great potential in the development of new electrocatalysts for renewable fuel production. Here, we engineered the intact proteinaceous shell of the carboxysome, a self-assembling protein organelle for CO<sub>2</sub> fixation in cyanobacteria and proteobacteria, and sequestered heterologously produced [NiFe]-hydrogenases into the carboxysome shell. The protein-based hybrid catalyst produced in <i>E. coli</i> shows substantially improved hydrogen production under both aerobic and anaerobic conditions and enhanced material and functional robustness, compared to unencapsulated [NiFe]-hydrogenases. The catalytically functional nanoreactor as well as the self-assembling and encapsulation strategies provide a framework for engineering new bioinspired electrocatalysts to improve the sustainable production of fuels and chemicals in biotechnological and chemical applications.

Item Type: Article
Uncontrolled Keywords: Cyanobacteria, Escherichia coli, Hydrogen, Hydrogenase, Catalysis
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 21 Feb 2023 14:52
Last Modified: 12 Apr 2023 09:40
DOI: 10.1039/d2tb02781j
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