Glycoproteins Involved in Sea Urchin Temporary Adhesion

Ventura, Ines, Harman, Victoria ORCID: 0000-0002-0990-153X, Beynon, Robert J ORCID: 0000-0003-0857-495X and Santos, Romana (2023) Glycoproteins Involved in Sea Urchin Temporary Adhesion. MARINE DRUGS, 21 (3). 145-. ISSN 1660-3397, 1660-3397

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Abstract

Biomedical adhesives, despite having been used increasingly in recent years, still face a major technological challenge: strong adhesion in wet environments. In this context, biological adhesives secreted by marine invertebrates have appealing characteristics to incorporate into new underwater biomimetic adhesives: water resistance, nontoxicity and biodegradability. Little is still known about temporary adhesion. Recently, a transcriptomic differential analysis of sea urchin <i>Paracentrotus lividus</i> tube feet pinpointed 16 adhesive/cohesive protein candidates. In addition, it has been demonstrated that the adhesive secreted by this species is composed of high molecular weight proteins associated with N-Acetylglucosamine in a specific chitobiose arrangement. As a follow-up, we aimed to investigate which of these adhesive/cohesive protein candidates were glycosylated through lectin pulldowns, protein identification by mass spectroscopy and in silico characterization. We demonstrate that at least five of the previously identified protein adhesive/cohesive candidates are glycoproteins. We also report the involvement of a third Nectin variant, the first adhesion-related protein to be identified in <i>P. lividus</i>. By providing a deeper characterization of these adhesive/cohesive glycoproteins, this work advances our understanding of the key features that should be replicated in future sea urchin-inspired bioadhesives.

Item Type:	Article
Uncontrolled Keywords:	sea urchin, tube feet, bioadhesive, glycoproteins, biomimetic adhesive
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology Faculty of Health and Life Sciences > Tech, Infrastructure and Environmental Directorate
Depositing User:	Symplectic Admin
Date Deposited:	17 Apr 2023 09:46
Last Modified:	07 Dec 2024 23:33
DOI:	10.3390/md21030145
Open Access URL:	https://doi.org/10.3390/md21030145
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3169603