Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population



Greenland-Bews, Caitlin, Byrne, Rachel LL, Owen, Sophie II, Watkins, Rachel LL, Bengey, Daisy ORCID: 0000-0002-0045-0218, Buist, Kate, Clerkin, Karina, Escadafal, Camille, Finch, Lorna SS ORCID: 0000-0002-8167-8766, Gould, Susan
et al (show 12 more authors) (2023) Evaluation of eight lateral flow tests for the detection of anti-SARS-CoV-2 antibodies in a vaccinated population. BMC INFECTIOUS DISEASES, 23 (1). 110-.

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Abstract

<h4>Background</h4>Rapid determination of an individual's antibody status can be beneficial in understanding an individual's immune response to SARS-CoV-2 and for initiation of therapies that are only deemed effective in sero-negative individuals. Antibody lateral flow tests (LFTs) have potential to address this need as a rapid, point of care test.<h4>Methods</h4>Here we present a proof-of-concept evaluation of eight LFT brands using sera from 95 vaccinated individuals to determine sensitivity for detecting vaccination generated antibodies. Samples were analysed on eight different brands of antibody LFT and an automated chemiluminescent microparticle immunoassay (CMIA) that identifies anti-spike antibodies which was used as our reference standard.<h4>Results</h4>All 95 (100%) participants tested positive for anti-spike antibodies by the chemiluminescent microparticle immunoassay (CMIA) reference standard post-dose two of their SARS-CoV-2 vaccine: BNT162b2 (Pfizer/BioNTech, n = 60), AZD1222 (AstraZeneca, n = 31), mRNA-1273 (Moderna, n = 2) and Undeclared Vaccine Brand (n = 2). Sensitivity increased from dose one to dose two in six out of eight LFTs with three tests achieving 100% sensitivity at dose two in detecting anti-spike antibodies.<h4>Conclusions</h4>These tests are demonstrated to be highly sensitive to detect raised antibody levels in vaccinated individuals. RDTs are low cost and rapid alternatives to ELISA based systems.

Item Type: Article
Uncontrolled Keywords: Antibody, Diagnostics, COVID-19, Vaccination, Serology
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 19 Apr 2023 16:57
Last Modified: 19 Apr 2023 16:57
DOI: 10.1186/s12879-023-08033-1
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3169781