A robust method for performing ¹ H-NMR lipidomics in embryonic zebrafish

Morgan, Rhiannon ORCID: 0000-0001-5257-6480, Walmsley, Gemma ORCID: 0009-0000-0727-8265, Peffers, Mandy ORCID: 0000-0001-6979-0440, Barrett-Jolley, Richard ORCID: 0000-0003-0449-9972 and Phelan, Marie (2021) A robust method for performing ¹ H-NMR lipidomics in embryonic zebrafish [Preprint]

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Abstract

The embryonic zebrafish is an ideal system for lipid analyses with relevance to many areas of bioscience research, including biomarkers and therapeutics. Research in this area has been hampered by difficulties in extracting, identifying and quantifying lipids. We employed 1 H-NMR at 700MHz to profile lipids in developing zebrafish embryos. The optimal method for lipidomics in embryonic zebrafish incorporated rapid lipid extraction using chloroform and an environment without oxygen depletion. Pools of 10 embryos gave the most acceptable signal-to-noise ratio, and the inclusion of chorions in the sample had no significant effect on lipid abundances. Embryos, bisected into cranial (head and yolk sac) and caudal (tail) regions, were compared by principal component analysis and analysis of variance. The lipid spectra (including lipid annotation) are available in the public repository MetaboLights (MTBLS2396).

Item Type:	Preprint
Uncontrolled Keywords:	3205 Medical Biochemistry and Metabolomics, 3401 Analytical Chemistry, 32 Biomedical and Clinical Sciences, 34 Chemical Sciences
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	17 May 2023 15:26
Last Modified:	29 May 2026 06:51
DOI:	10.1101/2021.05.26.445770
Open Access URL:	https://www.biorxiv.org/content/10.1101/2021.05.26...
Related Websites:	Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3170425
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