Community-developed checklists for publishing images and image analyses

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Schmied, Christopher, Nelson, Michael S, Avilov, Sergiy, Bakker, Gert-Jan, Bertocchi, Cristina, Bischof, Johanna, Boehm, Ulrike, Brocher, Jan, Carvalho, Mariana T, Chiritescu, Catalin et al (show 44 more authors) , Christopher, Jana, Cimini, Beth A, Conde-Sousa, Eduardo, Ebner, Michael, Ecker, Rupert, Eliceiri, Kevin, Fernandez-Rodriguez, Julia, Gaudreault, Nathalie, Gelman, Laurent, Grunwald, David, Gu, Tingting, Halidi, Nadia, Hammer, Mathias, Hartley, Matthew, Held, Marie ORCID: 0000-0003-0118-5898, Jug, Florian, Kapoor, Varun, Koksoy, Ayse Aslihan, Lacoste, Judith, Le Dévédec, Sylvia, Le Guyader, Sylvie, Liu, Penghuan, Martins, Gabriel G, Mathur, Aastha, Miura, Kota, Montero Llopis, Paula, Nitschke, Roland, North, Alison, Parslow, Adam C, Payne-Dwyer, Alex, Plantard, Laure, Ali, Rizwan, Schroth-Diez, Britta, Schütz, Lucas, Scott, Ryan T, Seitz, Arne, Selchow, Olaf, Sharma, Ved P, Spitaler, Martin, Srinivasan, Sathya, Strambio-De-Castillia, Caterina, Taatjes, Douglas, Tischer, Christian and Jambor, Helena Klara (2023) Community-developed checklists for publishing images and image analyses Nature Methods, 21 (2). pp. 170-181. ISSN 1548-7091, 1548-7105

Text ChecklistsImagePublishing_2023-07_06_ArxivV2.pdf - Author Accepted Manuscript Download (1MB) | Preview

Abstract

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.

Item Type:	Article
Uncontrolled Keywords:	Microscopy, Reproducibility of Results, Image Processing, Computer-Assisted, Publishing, Checklist
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Tech, Infrastructure & Env Directorate
Depositing User:	Symplectic Admin
Date Deposited:	15 Sep 2023 09:09
Last Modified:	28 Feb 2026 23:46
DOI:	10.1038/s41592-023-01987-9
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3172778
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