Expression of CYP2B6 Enzyme in Human Liver Tissue of HIV and HCV Patients

Obradovic, Bozana, Roberts, Owain, Owen, Andrew ORCID: 0000-0002-9819-7651, Milosevic, Ivana, Milic, Natasa, Ranin, Jovan and Dragovic, Gordana
(2023) Expression of CYP2B6 Enzyme in Human Liver Tissue of HIV and HCV Patients. MEDICINA-LITHUANIA, 59 (7). 1207-.

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<i>Background and Objectives</i>: Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) infections present significant public health challenges worldwide. The management of these infections is complicated by the need for antiviral and antiretroviral therapies, which are influenced by drug metabolism mediated by metabolic enzymes and transporters. This study focuses on the gene expression of <i>CYP2B6</i>, <i>CYP3A4</i>, and <i>ABCB1</i> transporters in patients with HIV, HCV, and HIV/HCV co-infection, aiming to assess their potential association with the choice of therapy, patohistological and clinical parameters of liver damage such as the stage of liver fibrosis, serum levels of ALT and AST, as well as the grade of liver inflammation and other available biochemical parameters. <i>Materials and Methods</i>: The study included 54 patients who underwent liver biopsy, divided into HIV-infected, HCV-infected, and co-infected groups. The mRNA levels of <i>CYP2B6</i>, <i>CYP3A4</i>, and <i>ABCB1</i> was quantified and compared between the groups, along with the analysis of liver fibrosis and inflammation levels. <i>Results</i>: The results indicated a significant increase in <i>CYP2B6</i> mRNA levels in co-infected patients, a significant association with the presence of HIV infection with an increase in <i>CYP3A4</i> mRNA levels. A trend towards downregulation of <i>ABCB1</i> expression was observed in patients using lamivudine. <i>Conclusions</i>: This study provides insight into gene expression of <i>CYP2B6 CYP3A4</i>, and <i>ABCB1</i> in HIV, HCV, and HIV/HCV co-infected patients. The absence of correlation with liver damage, inflammation, and specific treatment interventions emphasises the need for additional research to elucidate the complex interplay between gene expression, viral co-infection, liver pathology, and therapeutic responses in these particular patients population.

Item Type: Article
Uncontrolled Keywords: ABCB1, DAA, gene expression, HCV, HIV, HIV co-infection, metabolic enzymes, metabolic transporters
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 29 Sep 2023 09:26
Last Modified: 29 Sep 2023 09:26
DOI: 10.3390/medicina59071207
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